Microscopy Systems

Multi-Photon Imaging System




Scientifica Multiphoton Imaging System


Scientifica’s award-winning two-photon imaging system comes with a galvanometer scan head for the greatest flexibility to direct the laser to any position in the field of view.

'Galvo' mirrors offer the benefit of variable scanning speeds, adjustable to suit each individual application. Highly flexible scan patterns such as arbitrary line scans, spiral scans or patterned point scanning (e.g. for uncaging experiments) can easily be achieved.



Variable scanning speeds

Capture a specific region, improve speed and increase the amount of data collected. Rather than relying on averaging to improve signal-to-noise ratios, a reduced scan speed can achieve the same cell image morphology results while decreasing a sample’s exposure to laser light. This reduces bleaching and tissue damage.

Deep imaging

Outstanding images of the finest structures deep within your sample. Combine with optical sectioning to produce excellent three-dimensional models.

Region of interest scanning

Increase the yield and veracity of your results when studying small cellular structures, such as a spine or bouton, on a dendrite or axon.

Fast frame rates

The galvanometer scan mirrors facilitate frame rates of up to 15 frames per second for freely rotatable fields of view or 30 frames per second with bi-directional scanning.

Pioneering slim design

A compact footprint and flexible design as the basis for the Multiphoton system allows easy integration with other techniques such as electrophysiology.

Easily convert between in vivo and in vitro

The SliceScope's unique removable sub-stage, increasing the range of experiments which can be carried out on one rig.

Uniform “spot size”

The relay lens system within the Scientifica scan head achieves highly accurate imaging of small structures and prevents vignetting (or loss of signal) at the edge of the image.

Scanning mirrors
Scanning mirrors
3 x 5 mm 8315KL scanning mirrors (MicroMax 671HP Drivers)
Beam input height
Beam input height
MP-1000: 198 mm
 
 
MP-2000: 280.6 mm
Maximum beam input diameter
Maximum beam input diameter
<3 mm
Relay lens expansion
Relay lens expansion
1x
Beam expansion
Beam expansion
MP-1000: 3.3X
 
 
MP-2000: 6.7X
Beam diameter at back aperture
Beam diameter at back aperture
MP-1000: <10 mm
 
 
MP-2000: <20.1 mm
Lens coating
Lens coating
700-1200 nm (Rav <0.5%)
Maximum scan speed
Maximum scan speed
2 kHz in bidirectional scan mode (4 fps @512x512 px), 1 kHz in unidirectional scanning mode (2 fps @512x512 px)
Maximum scan angles (galvo)
Maximum scan angles (galvo)
MP-2000: +/- 10 optical degrees
Maximum scan angles (scan head)
Maximum scan angles (scan head)
+/- 7.6 optical degrees
Galvo voltage/optical degree
Galvo voltage/optical degree
0.25 V
Turning mirror size
Turning mirror size
MP-1000: 26 x 36 x 3 mm
 
 
MP-2000: 45 x 64 x 6 mm
Turning mirror coating
Turning mirror coating
Protected silver (98% reflectivity)
Typical field of view
Typical field of view
MP-1000: ~800 µm2 (16x)*, ~750 µm2 (20x)*
 
 
MP-2000: ~300 µm2 (40x)*
Scan Control
Scan Control
SciScan, ScanImage 3.8.1
 
 
*May not be achievable if scanning at high speeds for prolonged periods of time