Microscopy Systems

VT-iSIM: High Speed Super Resolution

Visitech Visitech

VisiTech Internation has its headquarters in Sunderland, UK. The company’s roots can be traced back to Joyce Loebl Ltd, one of the pioneering companies in image processing technology, and Applied Imaging, a leading supplier of Life Science instrumentation. In January 1999, a management buy-out from Applied Imaging resulted in the creation of VisiTech International as an independent company.

Website: http://www.visitech.co.uk

VisiTech vT-iSIM
VisiTech VT-iSIM

VT-iSIM: High Speed Super Resolution


The new ground breaking high speed super resolution imaging system from VisiTech

This device produces super resolution images in real time at up to 1,000 frames per second. The VT-iSIM can image at spatial and temporal resolutions and does not require any specific fluorophores, immersion oils, objective lenses or even microscope frames to work. If you have a sample which fluoresces you can enhance your spatial and axial resolution by up to 2X regular for wide-field microscopy.

  • Super Resolution Imaging
  • Fast Scanning (>1000fps)
  • X,Y <125nm
  • Z<350nm
  • Low Photo Bleaching
  • Multi Point Scanning Technique
  • Pin Holes selectable from 10-64µm
  • Automated 3-Position Dichroic Changer
  • Up to 6 SS lasers from visible range (405, 445, 488, 514, 532, 561, 642)
  • Camera connection is via regular c-mount
  • Regular or High Speed 6-Position Emission Filter Changer
  • VTi’s VoxCell Scan Acquisition software, Meta-Morph & NIS Elements
Unique Advantages of VT-iSIM

World’s first Multi-Point Super Resolution Imaging System:

VT-iSIM is the world’s first Multi Point Super Resolution Imaging System. This enables parallel processing of emitted photons through our emission micro-lens array optic thus allowing super resolution images to be collected at up to 200fps at a pixel resolution of 1024x1024 and FOV of 66.56x66.56µm. Spatial resolution at this rate of acquisition is maintained at 125nm in X & Y and 350nm in Z.

Super resolution without computation:

VT-iSIM produces images beyond the diffraction limit in real time onto a 2 dimensional array detector such as an sCMOS camera chip. There is no requirement for post processing or image manipulation.

Parallel Multi-Colour Image Acquisition:

VT-iSIM can chromatically split the fluorescence emission by wavelength onto multiple array detectors or even onto a single array detector to enable parallel multi-colour image acquisition at spatial resolutions of 135x135x365 and temporal resolutions of up to 1KHz.

No specific imaging requirements:

VT-iSIM does not require any specific fluorophores, immersion oils, objective lenses or even microscope frames to work. If you have a sample which fluoresces you can enhance your spatial and axial resolution by up to 2X.

Comparison Table:

 STEDSTORM /
PALM
AiryScanSIMVT-iSIM
Multi Point/High Speed Super Resolution Imaging x x x x
"Real Time" Image Acquisition x x x
Parallel multi-colour imaging x x x x
Set-up free Super Resolution Imaging x x x

 

VisiTech VT-iSIM

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