Applied Scientific Instrumentation, Inc. (ASI) were stablished in 1990. Their product line of devices for microscope automation and imaging represents the best technology available. ASI develops Ultra Precise Motion Control Systems, Automation, Modular Microscopes, Complete Light Sheet Systems, and components, Imaging Accessories, Focusing Systems, and High Precision Motorized Stages.
ASI has developed a new form of light sheet microscopy with our collaborators within the scientific community. The system utilizes two water emersion objectives mounted at 45 degree angles above the sample on an inverted microscope, a third objective also can be used to view the sample from below. The top objectives are mounted on piezo positioning devices so precise image stacks can be obtained. The system utilizes micro-mirrors to generate the light sheet, and the complete system can be mounted on to an existing inverted microscope, or supplied with ASI’s Rapid Automated Modular Microscope system (RAMM). Some of the benefits of the system include:
Use conventional mounting /glass cover slips
Generate 3D volumes with isotropic resolution (330 nm in all directions)
Axial resolution is ~2x better than confocal- or spinning disk systems
Achieve a ~7-10 fold reduction in photobleaching
Acquisition rates up to 200 images per second or 2-5 volumes per second (roughly 10x faster than spinning-disk microscopes)
Compared to Bessel beam plane illumination approaches, diSPIM offers equivalent (or better) axial resolution (~330 nm), 10-100x faster volumetric imaging rates (0.5-1 s instead of 10-100 s), and the ability to image over ~10x more time points (~1000 instead of ~100), due to the significantly lower illumination dose employed in our experiments and the lack of extraneous illumination outside the focal plane
Microscope has multicolor capability, and has been tested successfully on cells cultured on cover slips, cells embedded in collagen gels, and nematode and zebrafish embryos.
Luxendo is a recent spin-off from EMBL in Heidelberg, commercializing advanced Single Plane Illumination Microscopes (SPIM). They launched the MuVi-SPIM and InVi-SPIM microscopes in 2016. Both microscopes avoid sample photo toxicity by sequentially illuminating a stack of small slices of an organism, allowing scientists to observe living organisms for extended periods of time without photo damage.
Invi-SPIM system is dedicated to live imaging and is conﬁgured as an inverted microscope, optimized for long-term 3D ﬂuorescence imaging of living specimens. Easy access to the sample chamber, maximized photo-efficiency, and short illumination times enable long-term imaging without harming live specimens and cell cultures, and facilitates early embryonic development imaging. The unique optical configuration, in addition to ultra-fast acquisition speeds, enables 3D reconstruction, tracking of cellular and subcellular positions, and morphological analysis in real time. It utilizes light sheet technology, and includes an adjustable light sheet which is flexible in sheet area and variable in thickness 2-8 µm.
Inverted microscope configuration
Primary system for "in toto Imaging" of Mammalian Development from Zygote to Blastocyst.
Easy access to Sample Chamber
Long-term 3D Fluorescence Imaging of Live Specimens
Sample chamber and immersion medium separated
Illumination objective: 10 x @ 0.3 NA, H2O immersion
Detection objective: 25 x @ 1.1 NA, H2O immersion
Small sample chamber volume
Autoclavable, biologically inert sample chamber
Accurate temperature control
Optional environmental control (CO2, O2, N2)
Easy sample accessibility for e.g. patch clamp, photoactivation/photoablation
MuVI-SPIM incorporates the latest sCMOS camera technology and optimized combinations of objective lenses to enable you to obtain the best from your sample. It provides four simultaneous orthogonal views on large living specimens, without the need for sample rotation, in order to dispel shadowing effects and facilitate long-term imaging at ultra-high acquisition speeds. The MuVi-SPIM is the fastest SPIM on the market for analysing dynamic processes, enabling 4 simultaneous views at 65 frames/sec. Ideal for applications such as developmental biology, embryonic development, marine biology, neurobiology, functional imaging, long time lapse imaging and large specimen.
Large Field of View
Very High Imaging Speed;
Great Sample Handling and Low Ototoxicity
High Sensitivity and Low Noise
360 Illumination and Detection
High-speed sCMOS camera Hamamatsu ORCA-Flash 4.0
Laser combiner with six laser positions
2x Nikon CHI Plan Fluor 10XW 0.3NA water immersion for illumination
2x Nikon CFI LWD 25XW 1.1NA water immersion for detection
Mounting Chamber with temperature control (15°C – 40°C)