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Optogenetics

Optogenetics

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Mightex Optogenetics Systems

Mightex Systems, with operations based in Toronto, Canada, and in the Silicon Valley, California, is a leading supplier of photonic products and services for a broad spectrum of applications including industrial imaging, bio-photonic solutions, LED illumination, machine vision, life & health sciences, and industrial test & measurement etc.

Website: https://www.mightexbio.com/

Mightex Optogenetics Systems

Polygon: Cellular-Resolution Pattern Illumination


Patterned Illumination Systems
Pattern Illuminator for Life Science Research

Mightex’s market-leading Polygon pattern illuminators provide precise spatiotemporal control of light with subcellular resolution, making it the perfect illumination tool for life science research.

  • Any Shape, Size, Timing, and Colour
  • Simultaneous Multi-Region Illumination
  • Subcellular Resolution
  • Compatible with Any Microscope

The Polygon uses digital mirror device (DMD) technology to illuminate multiple regions simultaneously. A DMD is composed of hundreds of thousands of micro-mirrors that can be individually turned on to reflect light onto the sample. Thus, you can control each mirror to control the area(s) of illumination and create any number of different sized patterns. The Polygon DMD device can be mounted into the infinity-path of any microscope.

Patterned Illumination Systems
Different Models Optimized for Life Science Applications

Mightex offers multiple Polygon models that have been designed with different features to meet the needs of a wide-range of applications that use patterned illumination. Please see below for all available Polygon models.

Polygon1000-G

The Polygon1000-G is a flexible solution for patterned illumination, as this patterned illuminator can be used with any lightsource (350-700nm) that accepts a 3mm core lightguide. Thus, the Polygon1000-G provides future flexibility for different wavelengths and lightsources, depending on your application.

  • Wavelengths: 350-700nm
  • Lightsource: lightguide-coupled
  • Interchangeable front tubes available for fine resolution or large field-of-view

Key Applications: Neuroscience Optogenetics, Cell Biology Optogenetics, Photoactivation, Photoconversion

Polygon1000-DL

The Polygon1000-DL is a flexible solution for large field of view or high-power patterned illumination applications, as this patterned illuminator can be used with any fiber-coupled lightsource (400-700nm), such as high-power lasers. This Polygon1000 model has been designed for high-power applications, such as in vivo optogenetics.

  • Wavelengths: 400-700nm
  • Lightsource: fiber-coupled
  • Interchangeable front tubes available for fine resolution or large field-of-view

Key Applications: In Vivo Optogenetics, Cortex-Wide Optogenetics, Photoactivation

Powerful User-Friendly Polyscan Software for Life Science Experiments

Mightex’s PolyScan software platform is bundled with every Polygon to help you execute sophisticated patterned illumination experiments for your research. It provides:

  • Easy to use GUI to draw and define illumination patterns
  • Arrange sequences of illumination patterns
  • Define temporal illumination parameters for you experiments
  • Synchronize illumination patterns with other lab equipment
Synchronize with Electrophysiology or Imaging Equipment

The Polygon comes with BNC connectorized TTL trigger input and output for easy synchronization with multiple lab equipment. Synchronize the Polygon with your motorized stage for field of view stitching, or synchronize your stimulation patterns with electrophysiology equipment.


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Mightex Optogenetics Systems

OASIS Implant – Freely-Behaving Cellular-Resolution Calcium Imaging and Optogenetics


OASIS In Vivo Macro & Microscope Platform
Understand the Link Between Neural Circuits and Behaviour

The OASIS Implant is a ground-breaking platform for simultaneous cellular-resolution optogenetics and calcium imaging in freely-behaving animals to probe complex neuronal networks in the deep-brain, cortex, and multiple brain regions, or spinal cord.

  • Freely-Behaving Calcium Imaging and Optogenetics
  • Cellular-Resolution Optogenetics
  • Multi-Region Investigation
  • Reconfigurable Platform
  • High-Quality Imaging with Scientific Cameras
A Different Approach to Integrated Freely-Behaving Calcium Imaging and Optogenetics

To offer neuroscientists the ability to stimulate and visualize individual neurons in freely-behaving animals, Mightex’s engineers went back to the drawing board and pioneered a new path in the in vivo calcium imaging field. Instead of miniaturizing and placing the microscope on the animal – thereby limiting the microscope’s features and the animal’s behaviour – we kept the microscope out of the animal’s head by making use of long and flexible imaging fibers.

How It Works

By keeping the microscope away from the animal’s head, the OASIS Implant accomplishes 2 main goals:

Oasis Implant, optogenetics
Whitepaper

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Mightex Optogenetics Systems

OASIS Macro – Cortex-Wide Imaging and Optogenetics System


OASIS In Vivo Macro & Microscope Platform
Mesoscopic Imaging and Targeted Optogenetics Platform

The OASIS Macro is a mesoscopic platform for all-optical targeted optogenetics and calcium imaging. The OASIS Macro enables researchers to simultaneously image the entire mouse cortex and perform targeted optogenetics (with Mightex’s market-leading Polygon pattern illuminator).

  • Large field of view for in vivo imaging and optogenetics
  • Targeted optogenetics
  • Reconfigurable platform
  • Designed for in vivo experiments
Reconfigurable In Vivo Mesoscopic Platform for Imaging and Optogenetics

Mightex’s OASIS Macro is designed as a reconfigurable optical mesoscope system for in vivo imaging and optogenetics. The final system can be tailored to performance requirements for unique applications. This can include major features such as adding a second and third illumination source, or a dual-camera imaging system, as well as fine details including compatibility features for certain cameras and objectives.

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Mightex Optogenetics Systems

OASIS Micro – In Vivo Cellular-Resolution Optogenetics and Imaging System


OASIS Micro
Microscope Imaging and Cellular-Resolution Optogenetics Platform

The OASIS Micro is a ground-breaking microscope platform for all-optical cellular-resolution optogenetics and imaging in small cortical or deep-brain areas of a head-fixed animal. Simultaneously image small cortical or deep brain regions and perform neuron-specific targeted optogenetics with the addition of Mightex’s Polygon pattern illuminator.

  • Small cortical area or deep-brain for in vivo imaging and optogenetics
  • Cellular-resolution optogenetics
  • Reconfigurable platform
  • Designed for in vivo experiments
Reconfigurable In Vivo Microscope Platform for Imaging and Optogenetics

Mightex’s OASIS Micro is designed as a reconfigurable optical microscope system for in vivo imaging and optogenetics. The final system can be tailored to performance requirements for unique applications. This can include major features such as adding a second and third illumination source, or a dual-camera imaging system, as well as fine details including compatibility features for certain cameras and objectives.

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Mightex Optogenetics Systems

BioLED Sources & Controllers


BioLED Sources
Microscopy LEDs
Microscope, Microscopy, LED

Mightex BLS-series BioLED light sources are modularized fully-customizable turn-key solutions for optogenetics, fluorescence excitation, and other biophotonics applications. Precisely-timed and high-intensity light pulses are required in optogenetics experiments to activate channelrhodopsins (ChR2, ChR1 etc.) and halorhodopsins (NpHR) in order to excite and inhibit neurons. To meet these requirements, Mightex has developed a proprietary “IntelliPulsing” technology to allow BLS-series sources to output significantly higher power in pulse mode than what the LEDs are rated for in CW mode.

  • High-power UV/VIS/NIR/white fiber-coupled LED’s
  • Interchangeable fiber with SMA connector
  • Compact, machined metal housing with integrated heat sink


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Fiber-Coupled LEDs
Fiber Coupled LED

Mightex BLS-series BioLED light sources are modularized fully-customizable turn-key solutions for optogenetics, fluorescence excitation, and other biophotonics applications. Precisely-timed and high-intensity light pulses are required in optogenetics experiments to activate channelrhodopsins (ChR2, ChR1 etc.) and halorhodopsins (NpHR) in order to excite and inhibit neurons. To meet these requirements, Mightex has developed a proprietary “IntelliPulsing” technology to allow BLS-series sources to output significantly higher power in pulse mode than what the LEDs are rated for in CW mode.

  • High-power UV/VIS/NIR/white fiber-coupled LED’s
  • Interchangeable fiber with SMA connector
  • Optional LED controllers


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Lightguide-Coupled LEDs
Lightguide-Coupled LEDs

Mightex BLS-series BioLED light sources are modularized fully-customizable turn-key solutions for optogenetics, fluorescence excitation, and other biophotonics applications. Precisely-timed and high-intensity light pulses are required in optogenetics experiments to activate channelrhodopsins (ChR2, ChR1 etc.) and halorhodopsins (NpHR) in order to excite and inhibit neurons. To meet these requirements, Mightex has developed a proprietary “IntelliPulsing” technology to allow BLS-series sources to output significantly higher power in pulse mode than what the LEDs are rated for in CW mode.

  • High output power
  • Broad wavelength selections in VIS, UV and NIR
  • Interchangeable liquid lightguides or fiber bundles


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Spotlight LEDs
Lightguide-Coupled LEDs

Mightex BLS-series precision LED spotlight employ the latest high-power LED technologies and a proprietary coupling optics to achieve the best beam uniformity. In conjunction with BioLED light source control modules, these LED sources can be used for a wide range of applications including optogenetics and other bio-photonics applications.

  • High output power
  • Uniform illumination spot with a highly delineated edge
  • Multi-element aspherical optics, Adjustable focus


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BioLED Controllers
Manual/Analog LED Controllers
Manual/Analog LED Controllers

Mightex BLS-1000-2, BLS-3000-2, BLS-13000-1, and BLS-18000-1 LED controllers are designed for driving Mightex LED sources. The controllers have two operation modes:

Manual Mode: the output current can be controlled by manually turning the knobs; or Trigger Mode: the output current can be controlled via an external analog input signal between 0 ~ 5V.

The control mode is selected with a slide switch on the front panel, and the factory default is “Manual” mode. BLS-1000-2 and BLS-3000-2 provide Maximum Current Selection DIP switches on the rear panel, which allow the user to set the maximum output current of the channel to 500/750/1,000mA for BLS-1000-2, and 1,000/2,000/3,000mA for BLS-3000-2, respectively. The factory default is set to 500mA for BLS-1000-2 and 1,000mA for BLS-3000-2, respectively. BLS-13000-1 and BLS-18000-1 has only one maximum current setting of 13,000mA and 18,000mA, respectively.

When the controller is set to “Trigger” mode, the output current is fully controlled by the input analog signal (0-5V)..


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Software/TTL LED Controllers
Software/TTL LED Controllers

Mightex BLS-SA/PL series BioLED control modules are modularized fully-customizable turn-key solutions for optogenetics, fluorescence excitation, and other biophotonics applications. Precisely-timed and high-intensity light pulses are required in optogenetics experiments to activate channel-rhodopsins (ChR2, ChR1 etc.) and halorhodopsins (NpHR) in order to excite and inhibit neurons. To meet these requirements, Mightex has developed a proprietary “IntelliPulsing” technology to allow BLS-series sources to output significantly higher power in pulse mode than what the LEDs are rated for in CW mode.

The control module features a linear LED driver design that eliminates light intensity ripples and oscillations often observed when low-cost buckpuck nonlinear drivers are used. Clean and highly repeatable pulses are critical to quantitative experiments. Both CW mode and pulse modes are supported. Time resolution of the control module is 20us and light intensity can be adjusted with 0.1% increments. Each driving channel on the control module has its own TTL trigger input. Rising edge, falling edge, and follower mode are supported in the trigger mode.

Mightex BioLED light source solutions come with a Windows-based operation software featuring an intuitive yet powerful graphic user interface. A software development kit (SDK) is also provided for user integration into environment such as Labview and Matlab.

An optional touch-screen control pad (P/N: BLS-PAD-000) is available, with which customers can control LEDs without a computer.


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I/O Control Module
I/O Control Module

Mightex BioLED Analog and Digital I/O Control Module is a system-level control solution to be used in conjunction with the BioLED Manual/Analog Controlled LED Controllers and with the Polygon Patterned Illuminator. It greatly extends the flexibility and ease of system-level set-up when working with Mightex’s Polygon systems.

The I/O Control Module features four (4) independent channels. Each channel has an external trigger input (TTL), an analog voltage output (0-5V) and a digital output (LVTTL). All I/O connectors are BNC-type to work seamlessly with laboratory equipment. Intensity waveform of up to four (4) high-power LED light sources is controlled by the I/O Control Module1, each can output light at a different wavelength (i.e. color). Up to thirty-two (32) different waveforms can be programmed onto the I/O Control Module via USB or RS232. Each waveform can be associated with any one of the four LED’s to form a catalog of up to 128 unique Color/Waveform pairs.

The I/O Control Module can be synchronized with Polygon Patterned Illuminator. Each illumination pattern can be synchronized with one of the 128 Color/Waveform pairs, and one can generate a complex sequence of illumination Pattern/Color/Waveform combinations quickly and precisely. The I/O Control Module thus provides a convenient means to simultaneously achieve spatial, spectral, and temporal control of the most advanced illumination system for optogenetics and other experiments.


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Scientifica Electrophysiology scientifica

Scientifica manufacture a fully-motorised, fixed stage, focusing nosepiece, upright microscope for use in Elecrophysiology

Website: https://www.scientifica.uk.com



Scientifica LASU – Optogenetics, PhotoStimulation and Uncaging System


Developed with VU University Amsterdam, the LASU (Laser Applied Stimulation and Uncaging) system is ideal for researchers engaging in optogenetics, uncaging or other photostimulation experiments.



Accurate and repeatable

The laser spot positioning is accurate and repeatable enabling fast experimental manipulation of biological systems.

Award-winning technology

The Galvo mirror driven hardware and optical components are based on the Scientifica award-winning two-photon system giving you complete confidence when choosing LASU

Simple Integration

The LASU system fits directly onto the Scientifica upright SliceScope microscope making it an easy upgrade to existing rigs.

Free space launched or fibre guided laser delivery

The choice either fibre or free-space launch optics means that you can choose which laser delivery method best suits your experimental setup.

Choice of laser wavelengths

LASU is able to deliver multiple laser wavelengths with a spot smaller than 2 microns (depending on the objective used). This provides greater experimental control than ‘full field’ stimulation.

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