Biomolecular Imaging
Azure Biosystems are focused on the development and commercialization of state-of-the-art technologies to support research in the life science community. Founded in Dublin, California, Azure Biosystems develops high-performance instruments for image capture and analysis as well as high-quality reagents optimized to support select imaging applications.
Website: https://www.azurebiosystems.com
Sapphire FL Biomolecular Imager
Unmatched Application Versatility and Performance
The Sapphire FL is the ultimate biomolecular imager for FLEXIBILITY. With customizable and user-changeable laser and filter modules, the Sapphire FL easily adapts to a lab's changing needs and advancing research. The Sapphire FL offers customizable and user-changeable optical modules, 5-1000 μm resolution scans, a Z-plane range from -1.0 to +6 mm, 5 anesthesia ports for imaging living animals, chemiluminescence detection through the Chemiluminescence Module, and much more. The new Azure Sapphire FL is the second generation of the ground breaking Azure Sapphire Imager.
Key features
-
24-bit Dynamic Range:
Extended dynamic range allows imaging of both bright and weak bands without experiencing saturation. -
5 Micron Resolution:
From Western blots and gels, to microscope slides and live animal imaging, the adjustable 5-1000 µm resolution ensures unparalleled sample and application flexibility. -
Custom Design Lasers and Filters:
No limits – easily swap lasers and filters to achieve the perfect system to image your sample -
Adjustable focus for sample thickness:
Whether imaging Western blots, gels, 96-well plates, or tissue slides, the adjustable Z-plane ensures optimal focus for your sample.
Sapphire Biomolecular Imager
The Next Generation Lab Imaging Platform
With most imaging systems a decision between application flexibility and image quality has to be made. However, the Sapphire Biomolecular Imager is a next generation laser scanning system which offers the best of both worlds; unparalleled flexibility with excellent sensitivity and image quality. Regardless of the imaging application the Sapphire Biomolecular Imager and the powerful Sapphire Capture software will deliver high quality quantifiable data.
Key features
- Up to four solid state lasers (488, 520, 658 and 784 nm) offering ultimate excitation sensitivity
- Photomultiplier tube (PMT) for fluorescence and phosphor imaging, avalanche photodiodes (APD) for near-infrared imaging and a CCD sensor for chemiluminescent and visible imaging
- Ultra-wide dynamic range for imaging and quantifying low and high abundance samples simultaneously
- Image resolution down to 10 microns for high-quality image analysis
- Full integration with Sapphire Capture and AzureSpot software programs for perfect imaging and accurate analysis every time
chemiSOLO
Your Personal Western Blot Imager
Introducing a new, personal chemiluminescent imager that delivers high-quality, quantitative chemiluminescent and visible protein gel imaging through a unique web-based control software
Key features
-
Space-efficient design:
Smaller than a microwave, with the footprint of a laptop, the chemiSOLO replaces darkrooms and bulky imagers -
Highly Sensitive Imaging:
Rapidly images chemiluminescent Western blots with femtogram sensitivity -
Unique Web-based Connection:
Control the chemiSOLO using your phone, tablet, Mac or PC, no downloads required -
Quantitative Data Acquisition:
Linear detection from the chemiSOLO allows for Western blot quantitation over a wide dynamic range without saturation
Azure Imaging Systems
Azure 600
The Ultimate Western Blot Imaging System
Your Western blot method shouldn’t be limited by your imaging system, and with the Azure 600 it’s not. Fluorescent detection offers excellent quantitation and multiplex capability. Chemiluminescent detection delivers the ultimate sensitivity for imaging low abundance proteins. Azure’s 600 offers both.
Azure Biosystems doesn’t restrict your choice of reagents, either. With no proprietary stains or dyes, you get the freedom to capture data any way you want.
Key features
- Two Channel Infrared (IR) Direct Detection – Laser diodes at 660nm and 785nm offer better sensitivity than LEDs or white light sources
- Three Channel RGB Excitation – For Cy5/Cy3/Cy2 applications -allows quantitation of two proteins (or modifications) and a loading control without stripping and re-probing
- Fast Chemiluminescent Detection without film. Place your sample in the tray and capture your image with a click of a button
- Capture all your gel and blot images on a single instrument. DNA, protein, colorimetric or photometric
- 4.8 OD Dynamic Range
- Applications include Colour Imaging, Blue Light, UV Light, RGB, Chemi , and NIR.
600® | The All-around Application Ace
NIR ✓
RGB ✓
Chemi ✓
Blue Light ✓
Colour Imaging ✓
UltraViolet ✓
Azure 500
Infrared Western Blot Imaging System
Two-channel NIR fluorescent detection enables multiplex Western blotting for more efficient experiments. Image and quantify two different proteins without needing to strip and re-probe your blot. Highly sensitive chemiluminescent detection delivers film-levels of performance without the time-consuming hassles and inconveniences of the darkroom.
And, as with all cSeries systems, the Azure 500 is intuitive and easy to use. Simply place your sample in the system and capture an image once with the click of a button—no need to expose your blot to film multiple times to get the best image. Want to detect three proteins at once? The Azure 500 can be upgraded in the field by adding 3-channel visible fluorescence for Cy2/Cy3/Cy5 multi-protein detection capability.
Key features
- Two-channel IR direct detection-laser diodes at 660nm and 785nm enable better sensitivity than LEDs or white light sources
- Fast chemiluminescent detection without film. Place your sample in the tray and capture your image with a click of a button
- Upgradeable with 3-channel visible fluorescent detection – the Azure 500 can be field upgraded to the 600 to image three proteins at once with Cy2/Cy3/Cy5 or similar dyes
- Capture all your gel and blot images on a single instrument. DNA, protein, colorimetric or photometric
- 4.8 OD Dynamic Range
- Applications: Colour Imaging, Blue Light, UV Light, Chemi
500® | The Ultimate IR Imaging System
NIR ✓
Chemi ✓
Blue Light ✓
Colour Imaging ✓
UltraViolet ✓
Upgradeable ✓
Azure 400
Visible Fluorescent Western Blot Imaging System
The Azure 400 is a visible fluorescent imaging system that can also perform chemiluminescent detection at the same sensitivity as film. With three-channel RGB excitation for Cy5/Cy3/Cy2 fluorescent Western blot applications, you can quantify three proteins on a single blot (or two proteins and a loading control) without stripping and re-probing.
The Azure 400 also enables highly sensitive chemiluminescent detection so you can say goodbye to the hassles of film and darkroom chemicals—just place your sample in the system and capture an image with the click of a button.
Key features
- Three channel RGB excitation for Cy5/Cy3/Cy2 applications-allows quantitation of two proteins (or modifications) and a loading control without stripping and re-probing
- Fast chemiluminescent detection without the hassles and inconveniences of film
- Single, multi-functional instrument can image and quantify all of your gels and blots-DNA, protein, colorimetric and photometric
- Upgradeable to two-channel NIR fluorescent detection to meet future lab needs. The Azure 400 can be field upgraded to the 600 to image samples in the NIR
- 4.8 OD Dynamic Range
- Applications include Colour imaging, Blue Light, UV Light, Chemi and RGB.
400® | The Visible Fluorescence Virtuoso
RGB ✓
Chemi ✓
Blue Light ✓
Colour Imaging ✓
UltraViolet ✓
Upgradeable ✓
Azure 300
Chemiluminescent Western Blot Imaging System
Get digital chemiluminescent detection at a sensitivity equal to film but without all the hassles. Simply place your Western blot into the system and acquire the image with the click of a button.
Key features
- Compact design-with a footprint of 38cm W X 55cm H X 36cm D, the small size translates into more room on your bench
- Fully upgradable-add visible and/or NIR fluorescent detection capabilities as your needs evolve
- 4.8 OD Dynamic Range
- Applications Colour Imaging, Blue Light, UV Light and Chemi
300® | The Darkroom Eliminator
Chemi ✓
Blue Light ✓
Colour Imaging ✓
UltraViolet ✓
Upgradeable ✓
Azure 200
Gel Imaging Workstation
Easily image DNA and protein gels images without a darkroom with the Azure 200. Use your choice of dyes or stains and the system automatically selects the light source and filters for you—UV for Ethidium bromide-stained DNA gels, blue light for SYBR®Safe or similar dyes, white light for Silver stain or Coumassie Blue.
Key features
- Compact design-38cm W X 55cm H X 36cm D, keep more space free on your bench
- Fully upgradable-add fluorescent or chemiluminescent Western detection capabilities as your needs evolve
- Applications include Colour Imaging, Blue Light and UV Light.
200® | The Gel Imaging Workhorse
Blue Light ✓
Colour Imaging ✓
UltraViolet ✓
Upgradeable ✓
AzureSpot Analysis Software
Advanced Analysis for 1D Gels and Blots
AzureSpot helps you interpret your data easily and accurately. Analyze your gels and blots in either manual or automatic modes. AzureSpot can automatically detect lanes and bands, even on distorted gels, and apply your selected method of background correction. Visualize intensity plots by lane (sample) and channel (probe) to get detailed information about your proteins of interest.
The integrated tutorial helps you get the most information out of your analyses. Generate a PDF report of your experiment when you are done. You can also export your data in many formats to other applications (e.g. Excel) for further analysis.
AzureSpot can
- Perform 1D densitometry
- Automatically detect lanes and bands
- Correct for background
- Calculate molecular weights
- Quantify fluorescent signals
- Annotate your results
- Visualize multiplex analyses
- Export reports
Key features
- Integrated tutorial
- PDF report generator
- Lane creation tools
- Molecular size/pI calibration
- Quantity calibration toolbox
- Module for easy shape selection/deselection
- Wide range of data fields to display in measurements
- Annotation tools for comments and highlighting of image
Hardware and Software Requirements’
Operating Systems: Windows 10, Windows 8, Windows 7 and Windows Vista Processor 1.4 GHz, Memory Minimum 256MB, recommended 512MB Free Hard Disk Space Recommended 5GB.
Real-time PCR systems: Azure Cielo 3 & Azure Cielo 6
The Azure Biosystems™ Cielo™ 3 and 6 real-time PCR Systems are designed to offer smart protocols, high quality data, robust durability and an intuitive user experience.
Their novel fiber optic detection system allows for enhanced sensitivity and speed. 3 or 6 standard channels let you analyze a variety of commercially available dyes, already in your workflow. Onboard software operated by a 10.2″ touchscreen minimizes footprint and saves you bench space.
Easily connect to your Cielo via Wi-Fi, ethernet, USB or LAN. Get notified after each completed run and even remotely access protocols and files.
Applications
- Quantitative and qualitative gene expression analysis
- miRNA analysis
- Genetic mapping
- Genetic fingerprinting
- NGS library quantification
- 3 or 6 channel multiplex ability
- Pathogen quantification
Key features
- Tested durability up to 1000 repeat experiments. 1 year standard warranty
- Marlow Peltier heating block with 12 gradient temperature zones
- 6.0 °C/sec ramp rate
- 96-well format for semi-skirted or un-skirted plates
- Fiber optic detection for 16-wells simultaneously
- 3 or 6 optical channels for multiplexing
- Sensitivity up to 1 copy of amplified DNA, 10 logs of dynamic range
- Open source chemistry, including FAST. ~20 minute run-time
- Easily export data via Wi-Fi, ethernet, USB or LAN. Get notified after each completion
| Dyes/Probes | Azure Cielo 3 | Azure Cielo 6 |
|---|---|---|
| SYBR™ Green, EvaGreen™, FAM™ | ✓ | ✓ |
| VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor® Orange 560, | ✓ | ✓ |
| TAMRA™ | ✓ | |
| ROX™, TEX®615, CAL Fluor® Red 610 | ✓ | |
| CY®5, Quasar®670, Liz® Mustang Purple® | ✓ | ✓ |
| CY® 5.5 | ✓ |