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Electrophysiology

Cardiac Electrophysiology

MapplingLab based in Oxford UK, manufactures data acquisition and scientific equipment for cardiac electrophysiological research.

Website: https://mappinglab.com

Electrical Mapping Systems

Electrical Mapping Systems

Electrical Mapping Systems

The Electrical Mapping Systems (EMS) are equipped with the new generation of Multi-Electrode Arrays (MEAs) and high-performance amplifiers. The advanced EMapping software facilitates fast recordings of field potentials from in vitro, in vivo, and ex vivo cardiac samples. The electrical activities and conductive information can be studied in great details at tissue level. The precision of data acquisition system makes it possible to accurately detect abnormalities on ion channels. It also can be grouped with other devices such as ventricular or coronary pressure probe, monophasic action potential system (MAP), myocardial tension, perfusion temperature and the optical mapping system. These features immensely empower cardiac electrophysiological researchers to understand better about mechanisms of cardiac arrhythmias. Our system is also an ideal candidate for efficiently screening new anti-arrhythmic drugs and testing drug toxicity.

Applicable animals Zebrafish, mice, rats, guinea pigs, rabbits, dogs, sheep, pigs, monkeys.

Applicable tissues: cardiac slices and tissues, conductive tissues including sinoatrial node, atrioventricular node, Purkinje fibers, iPSC cells as well as in vivo and ex vivo heart, may also applicable to brain in vivo , brain slices, gastrointestinal tissues and uterus with customized system.

Features
  • High performance amplifiers and analog-digital converters (ADCs).
  • Advanced acquisition and intuitive analysis software.
  • Data transmission: USB or PXI fibre optic transmission.
  • Various size and layout.
  • Up to 16 additional channels.
  • Multiple input/output (I/O) connectors.
  • USB CCD camera.
  • 12V DC power supply.
Software

EMapping software includes: EMapRecord and EMapScope. The EMapRecord is an advanced acquisition software for recording up to 256 multi-channel data, whereas EMapScope is a high performance analysis software with an user-friendly interface.

EMapRecord-Acquistion

EMapScope-Analysis

Hardware

Amplifiers: EMS64-USB-1003, EMS128-PXI-1002 and EMS256-PXI-1001.

EMS64-USB-1003

EMS128-PXI-1002

EMS256-PXI-1001

Accessories and Parts

Pen MEAs
Pen MEAs

These electrode arrays are typically arranged as 32, 36 and 64 grids for recording electrical signals. Obtained signals will be amplified by a filter amplifier and sent to a data acquisition computer. MappingLab offers microelectrodes with different sizes to match the size of samples. They are applicable to record electrical activation and excitation of perfused cardiac tissues in chamber or a whole heart from mouse, rat, Guinea pig, rabbit, sheep and dog in vivo and ex vivo. It can be stably mounted on the ball-joint holder to probe the samples from multiple angles. They are suitable to measure direction and velocity of conduction system as well as to monitor electrical activities from cultured pacing tissues. More excitedly, they can be used in drug testing and safety.

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Flexible MEAs
Flexible MEAs

The flexible MEAs are gold or silver wires embedded in polyimide (PI) film with a thickness of 0.01mm to 0.1mm. Due to its adhesive and stretchable nature of polyimide, one of the advantages of using flexible MEAs is to have a close contact between the tissue and the electrodes, therefore, it makes them a perfect candidate for in vivo studies in heart and brain.

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Glass Microelectrode Arrays
Glass Microelectrode Arrays

The Glass Microelectrode Arrays are a powerful tool to provide a simplified model for cardiac and neuronal network research. Since the cells grow in a monolayer on a glass embedded with microelectrode arrays, therefore it is easy to monitor and manipulate cells with desired stimulating sequences in a defined area. Multiple measurements can be easily achieved over a long period of culturing. More excitedly, the network between cardiac and neuronal electrical activities can be studied in a simple environment.

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32 to 64 Channel Converter
32 to 64 Channel Converter
  • Individually or collectively obtain 32 or 64 channels of analog signals.
  • Conveniently convert two individuals 32 channels to 64 channels recording configuration for simultaneously recording of two different regions electrical activities.
  • Flexible and user-friendly design.

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CCD Camera
CCD Camera
  • 8 Megapixel high resolution camera.
  • Simultaneously image important steps and details during experiment such as settings, configurations and the position of electrode and the state of cardiac tissues.
  • Vividly review experimental conditions

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Multi-electrode Probe Connector
Multi-electrode Probe Connector
  • This adapter is designed to accommodate different types of glass MEAs. It facilitates to make recordings from cultured cardiac cells and tissue slices.
  • It can be safely kept in humid environment such as laboratory incubator for a long period.
  • The removable ball joint holder is handy to connect a perfusing line for samples and to cover metal mesh to shield interference for recordings.

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Optical Mapping Systems

Optical Mapping Systems

Optical Mapping Systems

MappingLab Ltd provides standard OMS-PCIE-1001, OMS-PCIE-1002 and OMSPCIE-2002 system as well as tailor-made systems. Dual signal optical mapping systems are suitable to record cardiac membrane potential and calcium imaging simultaneously. Our latest optical mapping system is equipped with the back Illuminated scientific CMOS cameras with 95% quantum efficiency (QE) and pixel size of 6.5 μm x 6.5 μm to 11 μm x 11 μm. It has a high speed and low noise acquisition with a dynamic range up to 50,000:1(94 dB). The optical axis can be constructed either vertically or horizontally. There are various I/O connectors for helping researchers to set up the communication system. A 8-channel TTL controller is designed for synchronizing devices such as camera, LED light controller, stimulator, ECG, MAP, pressure and temperature probe with the optical mapping system. The OMapRecord/Scope is an advanced data acquisition and analysis software which has an user-friendly intuitive interface and highly efficient data management.

Key Imaging Features
  • Detect area: 5 to 30 cm
  • Maximum resolution: 512*512 pixels.
  • Dynamic range up to 50,000:1(94 dB)
  • Sampling rate up 3.5 kHz with resolution 32*32 pixels.
  • ROI frame rate: up to 3.5 kHz.
  • 12 bit depth per pixel.
  • TTL synchronization precision: higher than 100μs.
  • Time-lapse imaging.
  • Customized ancillary devices
  • USB 3.0 or PCIE
Software

Windows 7, 8, 10 and Mac OS X

OMapRecord

OMapScope

Hardware
Pen MEAs

There is one CMOS camera fitted in OMS-PCIE-1001 and OMS-PCIE-1002. The difference between them is that the former can only acquire a single fluorescent signal,i.e. calcium or membrane potential whereas the latter can acquire two fluorescent signals simultaneously. However, OMS-PCIE-2002 has two CMOS cameras in the system to record calcium and membrane potential respectively and simultaneously. In principle, OMS-PCIE-2002 is the better choice for the dual signal recording due to higher resolution and less signal attenuation.

OMS-PCIE-1001

OMS-PCIE-1002

OMS-PCIE-2002

Ancillary Devices for Optical Mapping System
OMSC801: Optical System Contoller
OMSC801 Optical System Contoller

The Multi-Channel Signal Generator & Data Acquisition System OMSC801 can generate configurable pulse signals, meanwhile it can also acquire analog signals in real-time from other external amplifiers. It can effectively synchronize devices such as LED light controller, stimulator, ECG system, MAP, pressure, temperature probe and EMap system with the optical mapping system.

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LEDC-2001: LED Controller
LEDC-2001: LED Controller

The LEDC-2001 Controller is a device that controls the light strength from a LED source by using the adjustable flow currents.

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VCS-3001: Stimulator
VCS-3001: Stimulator

VCS-3001 stimulator is an essential device for cardiac researchers who need to give samples a repetitive stimulation to trigger electric activities. It has programmable parameters such as stimulus amplitude, pulse width and mode of stimulation.

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Heart / Tissue Perfusion Chamber
Heart / Tissue Perfusion Chamber

The small perfusion chamber is constructed for ex vivo experiments performing on isolated small hearts and organs from rodents as well as tissues in the controlled temperature conditions. It is designed for utilizing optical mapping recording, tissue dissection and stimulation that requires fast changes of solution.

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MappingLab Temperature Controllers

Temperature Controllers

PCTC1001 Temperature Controller
MappingLab PCTC1001 Temperature Controller

The temperature setting for PCTC1001 can be programmed. The controller is usually grouped with CH-16 Cubic Heater and TP Series Temperature Platform to heat up a perfusion system and maintain the temperature at a desired level in a working chamber. It also can be connected with TS-01 Test Point Sensor to monitor the temperature. It is an essential instrument for an electrophysiological research lab, especially for the lab equipped with patch clamp system.

Features
  • Easy to install and operate.
  • Temperatures display at real time.
  • Automatically store user’s setting.
  • Built-in thermistor fault protection from overheating.
  • External control is available.
  • High-accuracy temperature measurement

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PCTC2001 Temperature Controller
MappingLab PCTC2001 Temperature Controller

The PCTC2001 Temperature Controller can be optionally programmed to perform two heating modules. Firstly, it can be simply operated like a PCTC1001 Temperature Controller. In this module, it is grouped with both CH-16 Cubic Heater and TP-18 Temperature Platform to heat up a perfusion buffer and maintain the expected temperature at a desired level in a working chamber. Secondly, it can act like two PCTC1001 Temperature Controllers to heat up two independent solution lines. This function is fundamentally useful for researchers to perform a complicated experiment when more than one solution line is required.

Features
  • Two set of temperatures display in real time.
  • Easy to set up and operate.
  • Automatically store the user’s settings.
  • Built-in thermistor fault protection from overheating in the event of sensor failure.
  • Flexible temperature control.

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Accessories and Parts
CH-16 Cubic Heater
MappingLab CH-16 Cubic Heater

The CH-16 Cubic Heater is designed to warm up a solution and sustain the desired temperature at a constant level via a feedback loop.

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TP-18 Temperature Platform
MappingLab TP-18 Temperature Platform

The TP-18 Temperature Platform is designed to prevent heat loss from the thermal gradient and maintain a desired solution temperature.

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TS-03 Test Point Sensor
MappingLab TS-03 Test Point Sensor

The TS-03 Test Point Sensor is designed to detect the temperature of a desired point of interest. The testing temperature will be displayed on the front panel of the Temperature Controller.

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MappingLab Heart / Tissue Perfusion Systems

Heart / Tissue Perfusion Systems

MVC-801 Controller
MappingLab Heart / Tissue Perfusion Systems

8 pinch-valve perfusion system is designed for delivering experimental solutions in a controlled manner.

Features
  • 8 independent valves.
  • Versatile operation & free choice of any combination.
  • Flexible manual or computer control.
  • Programmable complex sequence.
  • High-precision solution delivery.
Compatibility
  • DrugDelivery
  • Windows 7, 8, 10

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Heart/Tissue Perfusion Chamber
MappingLab Heart / Tissue Perfusion Systems

The small perfusion chamber is constructed for ex vivo experiments performing on isolated small hearts and organs from rodents as well as tissues in the controlled temperature conditions. It is designed for utilizing optical mapping recording, tissue dissection and stimulation that requires fast changes of solution.

Features
  • Dimension: 15 x 15 x 6 cm,working chamber: 6 x 6 x 2 cm.
  • Chamber sizes can be customized.
  • Silicon chamber base.
  • 6 holes at the sides for fixing the cannula, solution tubes and stimulation electrode, etc. using mini ball joint clamps.
  • Controlled temperature by a water bath or a temperature controlling system.
  • Applicable to the solution bubbled with gas

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