Cardiac Electrophysiology
MapplingLab based in Oxford UK, manufactures data acquisition and scientific equipment for cardiac
electrophysiological research.
Website: https://mappinglab.com
Electrical Mapping Systems
The Electrical Mapping Systems (EMS) are equipped with the new generation of Multi-Electrode Arrays (MEAs) and
high-performance
amplifiers. The advanced EMapping software facilitates fast recordings of field potentials from in vitro, in vivo,
and ex vivo
cardiac samples. The electrical activities and conductive information can be studied in great details at tissue
level. The
precision
of data acquisition system makes it possible to accurately detect abnormalities on ion channels. It also can be
grouped with other
devices such as ventricular or coronary pressure probe, monophasic action potential system (MAP), myocardial
tension, perfusion
temperature and the optical mapping system. These features immensely empower cardiac electrophysiological
researchers to understand
better about mechanisms of cardiac arrhythmias. Our system is also an ideal candidate for efficiently screening new
anti-arrhythmic
drugs and testing drug toxicity.
Applicable animals Zebrafish, mice, rats, guinea pigs, rabbits, dogs, sheep, pigs, monkeys.
Applicable tissues: cardiac slices and tissues, conductive tissues including sinoatrial node,
atrioventricular
node, Purkinje fibers, iPSC cells as well as in vivo and ex vivo heart, may also applicable to brain in vivo , brain
slices,
gastrointestinal tissues and uterus with customized system.
Features
- High performance amplifiers and analog-digital converters (ADCs).
- Advanced acquisition and intuitive analysis software.
- Data transmission: USB or PXI fibre optic transmission.
- Various size and layout.
- Up to 16 additional channels.
- Multiple input/output (I/O) connectors.
- USB CCD camera.
- 12V DC power supply.
Software
EMapping software includes: EMapRecord and EMapScope. The EMapRecord is an advanced acquisition software for
recording up to 256
multi-channel data, whereas EMapScope is a high performance analysis software with an user-friendly interface.
EMapRecord-Record
EMapScope-Analysis
Hardware
Amplifiers: EMS64-USB-1003, EMS128-PXI-1002 and EMS256-PXI-1001.
EMS64-USB-1003
EMS64-USB-1003CS
EMS128-PXI-1002
EMS256-PXI-1001
Accessories and Parts
Pen MEAs
These electrode arrays are typically arranged as 32, 36 and 64 grids for recording electrical signals. Obtained
signals will
be amplified by a filter amplifier and sent to a data acquisition computer. MappingLab offers microelectrodes
with different
sizes to match the size of samples. They are applicable to record electrical activation and excitation of
perfused cardiac
tissues in chamber or a whole heart from mouse, rat, Guinea pig, rabbit, sheep and dog in vivo and ex vivo. It
can be stably
mounted on the ball-joint holder to probe the samples from multiple angles. They are suitable to measure
direction and velocity
of conduction system as well as to monitor electrical activities from cultured pacing tissues. More excitedly,
they can be used
in drug testing and safety.
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Flexible MEAs
The flexible MEAs are gold or silver wires embedded in polyimide (PI) film with a thickness of 0.01mm to 0.1mm.
Due to its
adhesive and stretchable nature of polyimide, one of the advantages of using flexible MEAs is to have a close
contact between
the tissue and the electrodes, therefore, it makes them a perfect candidate for in vivo studies in heart and
brain.
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More »
Glass Microelectrode Arrays
The Glass Microelectrode Arrays are a powerful tool to provide a simplified model for cardiac and neuronal
network research.
Since the cells grow in a monolayer on a glass embedded with microelectrode arrays, therefore it is easy to
monitor and
manipulate cells with desired stimulating sequences in a defined area. Multiple measurements can be easily
achieved over a long
period of culturing. More excitedly, the network between cardiac and neuronal electrical activities can be
studied in a simple
environment.
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32 to 64 Channel Converter
- Individually or collectively obtain 32 or 64 channels of analog signals.
- Conveniently convert two individuals 32 channels to 64 channels recording configuration for simultaneously
recording of two
different regions electrical activities.
- Flexible and user-friendly design.
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CCD Camera
- 8 Megapixel high resolution camera.
- Simultaneously image important steps and details during experiment such as settings, configurations and the
position of
electrode and the state of cardiac tissues.
- Vividly review experimental conditions
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Multi-electrode Probe Connector
- This adapter is designed to accommodate different types of glass MEAs. It facilitates to make recordings
from cultured
cardiac cells and tissue slices.
- It can be safely kept in humid environment such as laboratory incubator for a long period.
- The removable ball joint holder is handy to connect a perfusing line for samples and to cover metal mesh to
shield
interference for recordings.
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MappingLab Ltd provides standard OMS-PCIE-1001, OMS-PCIE-1002 and OMSPCIE-2002 system as well as tailor-made
systems. Dual signal
optical mapping systems are suitable to record cardiac membrane potential and calcium imaging simultaneously. Our
latest optical
mapping system is equipped with the back Illuminated scientific CMOS cameras with 95% quantum efficiency (QE) and
pixel size of 6.5
µm x 6.5 µm to 11 µm x 11 µm. It has a high speed and low noise acquisition with a dynamic range up to 50,000:1(94
dB). The optical
axis can be constructed either vertically or horizontally. There are various I/O connectors for helping researchers
to set up the
communication system. A 8-channel TTL controller is designed for synchronizing devices such as camera, LED light
controller,
stimulator, ECG, MAP, pressure and temperature probe with the optical mapping system. The OMapRecord/Scope is an
advanced data
acquisition and analysis software which has an user-friendly intuitive interface and highly efficient data
management.
Key Imaging Features
- Detect area: 5 to 30 cm
- Maximum resolution: 512*512 pixels.
- Dynamic range up to 50,000:1(94 dB)
- Sampling rate up 3.5 kHz with resolution 32*32 pixels.
- ROI frame rate: up to 3.5 kHz.
- 12 bit depth per pixel.
- TTL synchronization precision: higher than 100µs.
- Time-lapse imaging.
- Customized ancillary devices
- USB 3.0 or PCIE
Software
Windows 7, 8, 10 and Mac OS X
OMapRecord
OMapScope
Hardware
There is one CMOS camera fitted in OMS-PCIE-1001 and OMS-PCIE-1002. The difference between them is that the former
can only
acquire a single fluorescent signal,i.e. calcium or membrane potential whereas the latter can acquire two
fluorescent signals
simultaneously. However, OMS-PCIE-2002 has two CMOS cameras in the system to record calcium and membrane potential
respectively and simultaneously.
In principle, OMS-PCIE-2002 is the better choice for the dual signal recording due to higher resolution and less
signal
attenuation.
OMS-PCIE-1001
OMS-PCIE-1002
OMS-PCIE-2002
Ancillary Devices for Optical Mapping System
OMSC801: Optical System Contoller
The Multi-Channel Signal Generator & Data Acquisition System OMSC801 can generate configurable pulse
signals, meanwhile it can
also acquire analog signals in real-time from other external amplifiers. It can effectively synchronize devices
such as LED light
controller, stimulator, ECG system, MAP, pressure, temperature probe and EMap system with the optical mapping
system.
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LEDC-2001: LED Controller
The LEDC-2001 Controller is a device that controls the light strength from a LED source by using the adjustable
flow currents.
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More »
VCS-3001: Stimulator
VCS-3001 stimulator is an essential device for cardiac researchers who need to give samples a repetitive
stimulation to trigger
electric activities. It has programmable parameters such as stimulus amplitude, pulse width and mode of
stimulation.
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»
Heart / Tissue Perfusion Chamber
The small perfusion chamber is constructed for ex vivo experiments performing on isolated small hearts and
organs from rodents as
well as tissues in the controlled temperature conditions. It is designed for utilizing optical mapping
recording, tissue dissection
and stimulation that requires fast changes of solution.
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PCTC1001 Temperature Controller
The temperature setting for PCTC1001 can be programmed. The controller is usually grouped with CH-16 Cubic
Heater and TP Series
Temperature Platform to heat up a perfusion system and maintain the temperature at a desired level in a working
chamber. It
also can be connected with TS-01 Test Point Sensor to monitor the temperature. It is an essential instrument for
an electrophysiological
research lab, especially for the lab equipped with patch clamp system.
Features
- Easy to install and operate.
- Temperatures display at real time.
- Automatically store user’s setting.
- Built-in thermistor fault protection from overheating.
- External control is available.
- High-accuracy temperature measurement
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»
PCTC2001 Temperature Controller
The PCTC2001 Temperature Controller can be optionally programmed to perform two heating modules. Firstly, it
can be simply
operated like a PCTC1001 Temperature Controller. In this module, it is grouped with both CH-16 Cubic Heater and
TP-18 Temperature
Platform to heat up a perfusion buffer and maintain the expected temperature at a desired level in a working
chamber. Secondly, it can act
like two PCTC1001 Temperature Controllers to heat up two independent solution lines. This function is
fundamentally useful for
researchers to perform a complicated experiment when more than one solution line is required.
Features
- Two set of temperatures display in real time.
- Easy to set up and operate.
- Automatically store the user’s settings.
- Built-in thermistor fault protection from overheating in the event of sensor failure.
- Flexible temperature control.
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»
Accessories and Parts
CH-16 Cubic Heater
The CH-16 Cubic Heater is designed to warm up a solution and sustain the desired temperature at a constant
level via a
feedback loop.
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»
TP-20 Temperature Platform
The TP-20 Temperature Platform is designed to prevent heat loss from the thermal gradient and maintain a
desired solution
temperature.
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»
TS-03 Test Point Sensor
The TS-03 Test Point Sensor is designed to detect the temperature of a desired point of interest. The testing
temperature will
be displayed on the front panel of the Temperature Controller.
Learn
More
»
Heart / Tissue Perfusion Systems
MVC-801 Controller
8 pinch-valve perfusion system is designed for delivering experimental solutions in a controlled manner.
Features
- 8 independent valves.
- Versatile operation & free choice of any combination.
- Flexible manual or computer control.
- Programmable complex sequence.
- High-precision solution delivery.
Compatibility
- DrugDelivery
- Windows 7, 8, 10
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»
Heart/Tissue Perfusion Chamber
The small perfusion chamber is constructed for ex vivo experiments performing on isolated small hearts and
organs from
rodents
as well as tissues in the controlled temperature conditions. It is designed for utilizing optical mapping
recording, tissue
dissection and stimulation that requires fast changes of solution.
Features
- Dimension: 15 x 15 x 6 cm,working chamber: 6 x 6 x 2 cm.
- Chamber sizes can be customized.
- Silicon chamber base.
- 6 holes at the sides for fixing the cannula, solution tubes and stimulation electrode, etc. using mini ball
joint clamps.
- Controlled temperature by a water bath or a temperature controlling system.
- Applicable to the solution bubbled with gas
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»
