CoolLED
CoolLED designs and manufactures cutting edge LED illumination systems for researchers and clinicians using the latest LED technology.
The company has specialised in fluorescence microscopy since it introduced the first commercially available LED illumination system in 2004. LEDs are now the system of choice because they are more stable, longer lasting, and energy efficient than traditional mercury based illuminators as well as offering superior safety and environmental features.
Website: https://www.coolled.com
Models:
| 8-channel illumination and lightning fast control | Research Fluorescence Illumination | Simple White Light for everyday fluorescence | Everyday Controllable Illumination | Fast Controllable Illumination | LED Illumination for Fura-2 and common fluorophores | Single wavelength solution | Transmitted illumination |
pE-800 | pE-4000 | pE-300lite | pE-300white | pE-300ultra | pE-340fura | pE-100 | pT-100 |
pE-800 : 8-channel illumination and lightning fast control
- 8 selectable channels offers versatility with any channels selectable simultaneously at 100 %
- Individual control of selected LED wavelengths reduces background
- Broad spectrum from 365-740 nm, covering DAPI-Cy7
- Industry-leading <7 µs TTL triggering for high-speed imaging
- Sequence Runner for software control with speed of TTL
- Removable inline excitation filter holders: no moving parts for high-speed imaging
- Software, TTL, analogue control options for optimised illumination
- Optimised irradiance control in 1 % steps (2-100 %) and fine control from 0-2 %
- Light delivery via liquid Light Guide for maximum compatibility
The arrival of the pE-800 sees LEDs take centre stage as the widefield illumination method of choice. Featuring eight individually controllable LEDs and lightning fast TTL switching, the pE-800 delivers the highest quality data with minimum total cost of ownership. Versatile, intuitive and backed by CoolLED’s world-renowned support and a 36-month warranty, the pE-800 with liquid light guide delivery makes next generation LED illumination accessible to all.
pE-300 series : Regular fluorescence
- Broad spectrum
- Replaces mercury lamp
- TTL & USB Control ( pE-300white & pE-300ultra )
The pE-300 Series is a range of LED Illumination Systems for fluorescence, optogenetics, electrophysiology and high-speed microscopy applications. It comprises three systems providing a solution that covers a range of everyday fluorescence microscopy; from the simple pE-300lite through the classic pE-300white to the highly controllable pE-300ultra. The systems are unique in also being available as a Direct Fit model, which increases light intensity and reduces the cost to buy and run thanks to fewer consumables.
pE-340Fura : Fura-2 ratiometric calcium imaging + regular fluorescence
- Dedicated 340nm & 380nm outputs
- Broad white output ( 435nm to 645nm )
- Micro-second switching ( 340/380)
Utilising the successful pE-300 Series platform, the pE-340fura is a bespoke LED Illuminator for Fura-2 ratiometric calcium imaging, which also supports everyday fluorescence microscopy in a compact and affordable package.
The 340nm and 380nm LED illumination system provides the optimum excitation wavelengths for Fura-2-based calcium imaging allowing high-precision, stable, high-throughput imaging with video-rate time resolution.
pE-4000 : Research fluorescence
- 16 selectable wavelengths
- White mode
- Advanced mode
- TTL & USB control
The pE-4000 sets the standard as the universal illumination system for fluorescence microscopy. The system has 16 selectable LED wavelengths that can be matched to the filters and fluorophores of almost any microscope, making it the broadest spectrum of illumination available.
For the user who wants consistency and ease of use with simple on/off and intensity buttons, the pE-4000 can be operated as a ‘White’ illumination system.
For the user who wants total control over the excitation source to improve imaging, individual wavelengths settings can be selected and controlled in “Advanced” mode.
pE-100 : Screening fluorescence
- Single LED wavelength
- Simple to operate
- TTL control
The pE-100 series is a family of single-wavelength fluorescence LED illumination systems, which are compact and simple to use. Systems can be specified at any one of 20 different LED wavelengths, and are ideal for routine clinical screening, electrophysiology or basic research applications. Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%, with remote control available via a TTL trigger.
pT-100 : Transmitted illumination
- Halogen replacement
- Excellent colour rendering
- TTL control
The pT-100 is a compact and simple-to-use LED illumination system for transmitted light applications, including brightfield, darkfield, DIC, Dodt gradient contrast and phase contrast.
The system is available in four variants to bring the benefits of LEDs to modern laboratories. A broad white system provides a safer and convenient alternative to halogen by reducing harmful UV exposure to the eyes and is ideal for long hours spent at the microscope, while three narrow bandwidth options (525, 635 and 770 nm) optimise image quality through increased contrast.