Lasers & Light Sources

CoolLED

CoolLED

CoolLED designs and manufactures cutting edge LED illumination systems for researchers and clinicians using the latest LED technology.

The company has specialised in fluorescence microscopy since it introduced the first commercially available LED illumination system in 2004. LEDs are now the system of choice because they are more stable, longer lasting, and energy efficient than traditional mercury based illuminators as well as offering superior safety and environmental features.

Website: https://www.coolled.com

Models:

CoolLED Product Comparison »

Controllable white light for routine to advanced fluorescenceFast, 4-channel control8-channel illumination and lightning fast controlSupercharged calcium imaging and beyondResearch Fluorescence IlluminationSimple White Light for everyday fluorescenceEveryday Controllable IlluminationFast Controllable IlluminationLED Illumination for Fura-2 and common fluorophoresSingle wavelength solutionTransmitted illumination
pE-400
pE-800
pE-400max
pE-800
pE-800
pE-800
pE-800fura
pE-800 fura
pE-4000
pE-4000
pE-300lite
pE-300lite
pE-300white
pE-300white
pE-300ultra
pE-300ultra
pE-340fura
pE-340fura
pE-100
pE-100
pT-100
pT-100
CoolLEDControllable white light for routine to advanced fluorescence

pE-400 : Controllable white light for routine to advanced fluorescence

  • Global control of white light
  • Broad spectrum white light from 365-635 nm, ranging from DAPI through YFP to Cy5.
  • Seamless integration into compatible third party imaging software and cost-effective high-speed imaging with pE-6501 USB controlled TTL trigger box
  • < 10 µs global TTL triggering for camera synchronisation
  • Simple control pod for intuitive manual operation
  • Optimised irradiance control in 1% steps (0-100%)
  • Direct fit variant available for higher irradiance with selected/all major microscope models
  • Light delivery also available via liquid light guide.

Building on the success of the popular pE-300 Series, the pE-400 is a simple bright white light Illumination System and a controllable, cost-effective replacement for mercury and metal halide lamps. Four powerful LEDs offer intense, broad-spectrum coverage for the most popular fluorophores, ranging from DAPI through YFP to Cy5.

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CoolLEDControllable white light for routine to advanced fluorescence

pE-400max : Fast 4-channel controllable illumination for routine to advanced fluorescence

  • 4 channels can be individually controlled, with any simultaneously running at 100%
  • Broad spectrum white light from 365-635 nm, ranging from DAPI through YFP to Cy5.
  • Seamless integration into compatible third party imaging software and cost-effective high-speed imaging with pE-6501 USB controlled TTL trigger box
  • < 10 µs global TTL triggering for camera synchronisation
  • Optimised irradiance control in 1% steps (0-100%)
  • Sequence Runner for software control with speed of TTL
  • Optional inline excitation filter holders: no moving parts for high-speed imaging
  • Simple control pod for intuitive manual operation
  • pE-400max LightBridge graphical user interface (GUI) for intuitive digital operation
  • Direct fit variant available for higher irradiance with selected/all major microscope models
  • Light delivery also available via liquid light guide.

Building on the success of the popular pE-300 Series, the pE-400max is the most advanced Illumination System of the pE-400 Series. Four powerful LEDs can be individually controlled via software, TTL, Lightbridge GUI and manual control pod for demanding everyday fluorescence microscopy and optogenetics applications using the most popular fluorophores, ranging from DAPI through YFP to Cy5.

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CoolLED8-channel illumination and lightning fast control

pE-800 : 8-channel illumination and lightning fast control

  • 8 selectable channels offers versatility with any channels selectable simultaneously at 100 %
  • Individual control of selected LED wavelengths reduces background
  • Broad spectrum from 365-740 nm, covering DAPI-Cy7
  • Industry-leading <7 µs TTL triggering for high-speed imaging
  • Sequence Runner for software control with speed of TTL
  • Removable inline excitation filter holders: no moving parts for high-speed imaging
  • Software, TTL, analogue control options for optimised illumination
  • Optimised irradiance control in 1 % steps (2-100 %) and fine control from 0-2 %
  • Light delivery via liquid Light Guide for maximum compatibility

The arrival of the pE-800 sees LEDs take centre stage as the widefield illumination method of choice. Featuring eight individually controllable LEDs and lightning fast TTL switching, the pE-800 delivers the highest quality data with minimum total cost of ownership. Versatile, intuitive and backed by CoolLED’s world-renowned support and a 36-month warranty, the pE-800 with liquid light guide delivery makes next generation LED illumination accessible to all.

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CoolLED8-channel illumination and lightning fast control

pE-800fura : Supercharged calcium imaging and beyond

  • Broad spectrum from 340-635 nm tailored to popular calcium indicators and fluorophores
  • 8 selectable channels offer versatility with any channels selectable simultaneously at 100%
  • Industry-leading <7 µs TTL triggering for high-speed analysis
  • Affordable automation with Sequence Runner and a single TTL-out
  • Removable inline excitation filter holder: no moving parts for high-speed imaging
  • Software , TTL, analogue control options for optimised illumination
  • Optimised irradiance control in 1% steps (2-100%) and fine control from 0-2%
  • Light delivery via liquid Light Guide for maximum compatibility
  • Stable illumination minimises noise for precise calcium analysis and reduced false positives
  • High signal-to-noise for crisp images and quality data with less calcium indicator, reducing toxicity and cost
  • UV optimised optics maximises irradiance for Fura-2 and similar indicators

Building on the successful pE-800 Illumination System, the pE-800fura offers the most comprehensive calcium imaging system available. It also enhances a range of applications from pH monitoring and optogenetics to everyday fluorescence microscopy, combining lightning-fast switching with eight individually controllable channels. Backed by CoolLED’s world-renowned support and generous warranty, the pE-800fura presents the go-to illuminator for calcium imaging and beyond.

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CoolLEDRegular fluorescence LED light source

pE-300 series : Regular fluorescence

  • Broad spectrum
  • Replaces mercury lamp
  • TTL & USB Control ( pE-300white & pE-300ultra )

The pE-300 Series is a range of LED Illumination Systems for fluorescence, optogenetics, electrophysiology and high-speed microscopy applications. It comprises three systems providing a solution that covers a range of everyday fluorescence microscopy; from the simple pE-300lite through the classic pE-300white to the highly controllable pE-300ultra. The systems are unique in also being available as a Direct Fit model, which increases light intensity and reduces the cost to buy and run thanks to fewer consumables.

pE-300

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CoolLEDpE-340 Fura : Fura-2 ratiometric calcium imaging + regular fluorescence

pE-340Fura : Fura-2 ratiometric calcium imaging + regular fluorescence

  • Dedicated 340nm & 380nm outputs
  • Broad white output ( 435nm to 645nm )
  • Micro-second switching ( 340/380)

Utilising the successful pE-300 Series platform, the pE-340fura is a bespoke LED Illuminator for Fura-2 ratiometric calcium imaging, which also supports everyday fluorescence microscopy in a compact and affordable package.

The 340nm and 380nm LED illumination system provides the optimum excitation wavelengths for Fura-2-based calcium imaging allowing high-precision, stable, high-throughput imaging with video-rate time resolution.

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CoolLEDpE-4000 : Research fluorescence

pE-4000 : Research fluorescence

  • 16 selectable wavelengths
  • White mode
  • Advanced mode
  • TTL & USB control

The pE-4000 sets the standard as the universal illumination system for fluorescence microscopy. The system has 16 selectable LED wavelengths that can be matched to the filters and fluorophores of almost any microscope, making it the broadest spectrum of illumination available.

For the user who wants consistency and ease of use with simple on/off and intensity buttons, the pE-4000 can be operated as a ‘White’ illumination system.

For the user who wants total control over the excitation source to improve imaging, individual wavelengths settings can be selected and controlled in “Advanced” mode.

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CoolLEDpE-100 : Screening fluorescence

pE-100 : Screening fluorescence

  • Single LED wavelength
  • Simple to operate
  • TTL control

The pE-100 series is a family of single-wavelength fluorescence LED illumination systems, which are compact and simple to use. Systems can be specified at any one of 20 different LED wavelengths, and are ideal for routine clinical screening, electrophysiology or basic research applications. Operation is by a remote manual control pod with instant on/off and intensity control from 0-100%, with remote control available via a TTL trigger.

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CoolLEDpT-100 : Transmitted illumination

pT-100 : Transmitted illumination

  • Halogen replacement
  • Excellent colour rendering
  • TTL control

The pT-100 is a compact and simple-to-use LED illumination system for transmitted light applications, including brightfield, darkfield, DIC, Dodt gradient contrast and phase contrast.

The system is available in four variants to bring the benefits of LEDs to modern laboratories. A broad white system provides a safer and convenient alternative to halogen by reducing harmful UV exposure to the eyes and is ideal for long hours spent at the microscope, while three narrow bandwidth options (525, 635 and 770 nm) optimise image quality through increased contrast.

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