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In vivo & Biomolecular Imaging

In vivo & Biomolecular Imaging

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Azure Biosystems are focused on the development and commercialization of state-of-the-art technologies to support research in the life science community. Founded in Dublin, California, Azure Biosystems develops high-performance instruments for image capture and analysis as well as high-quality reagents optimized to support select imaging applications.


Website: https://www.azurebiosystems.com

Azure Biosystems Sapphire Biomolecular Imager

The Next Generation Lab Imaging Platform


With most imaging systems a decision between application flexibility and image quality has to be made. However, the Sapphire Biomolecular Imager is a next generation laser scanning system which offers the best of both worlds; unparalleled flexibility with excellent sensitivity and image quality. Regardless of the imaging application the Sapphire Biomolecular Imager and the powerful Sapphire Capture software will deliver high quality quantifiable data.

Key features
  • Up to four solid state lasers (488, 520, 658 and 784 nm) offering ultimate excitation sensitivity
  • Photomultiplier tube (PMT) for fluorescence and phosphor imaging, avalanche photodiodes (APD) for near-infrared imaging and a CCD sensor for chemiluminescent and visible imaging
  • Ultra-wide dynamic range for imaging and quantifying low and high abundance samples simultaneously
  • Image resolution down to 10 microns for high-quality image analysis
  • Full integration with Sapphire Capture and AzureSpot software programs for perfect imaging and accurate analysis every time
 

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Azure Biosystems Azure 600

Azure 600


The Ultimate Western Blot Imaging System

Your Western blot method shouldn’t be limited by your imaging system, and with the Azure 600 it’s not. Fluorescent detection offers excellent quantitation and multiplex capability. Chemiluminescent detection delivers the ultimate sensitivity for imaging low abundance proteins. Azure’s 600 offers both.

Azure Biosystems doesn’t restrict your choice of reagents, either. With no proprietary stains or dyes, you get the freedom to capture data any way you want.

Key features
  • Two Channel Infrared (IR) Direct Detection – Laser diodes at 660nm and 785nm offer better sensitivity than LEDs or white light sources
  • Three Channel RGB Excitation – For Cy5/Cy3/Cy2 applications -allows quantitation of two proteins (or modifications) and a loading control without stripping and re-probing
  • Fast Chemiluminescent Detection without film. Place your sample in the tray and capture your image with a click of a button
  • Capture all your gel and blot images on a single instrument. DNA, protein, colorimetric or photometric
  • 4.8 OD Dynamic Range
  • Applications include Colour Imaging, Blue Light, UV Light, RGB, Chemi ,br> and NIR.
 

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Azure Biosystems Azure 500

Azure 500


Infrared Western Blot Imaging System

Two-channel NIR fluorescent detection enables multiplex Western blotting for more efficient experiments. Image and quantify two different proteins without needing to strip and re-probe your blot. Highly sensitive chemiluminescent detection delivers film-levels of performance without the time-consuming hassles and inconveniences of the darkroom.

And, as with all cSeries systems, the Azure 500 is intuitive and easy to use. Simply place your sample in the system and capture an image once with the click of a button—no need to expose your blot to film multiple times to get the best image. Want to detect three proteins at once? The Azure 500 can be upgraded in the field by adding 3-channel visible fluorescence for Cy2/Cy3/Cy5 multi-protein detection capability.

Key features
  • Two-channel IR direct detection-laser diodes at 660nm and 785nm enable better sensitivity than LEDs or white light sources
  • Fast chemiluminescent detection without film. Place your sample in the tray and capture your image with a click of a button
  • Upgradeable with 3-channel visible fluorescent detection – the Azure 500 can be field upgraded to the 600 to image three proteins at once with Cy2/Cy3/Cy5 or similar dyes
  • Capture all your gel and blot images on a single instrument. DNA, protein, colorimetric or photometric
  • 4.8 OD Dynamic Range
  • Applications: Colour Imaging, Blue Light, UV Light, Chemi
 

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Azure Biosystems Azure 400

Azure 400


Visible Fluorescent Western Blot Imaging System

The Azure 400 is a visible fluorescent imaging system that can also perform chemiluminescent detection at the same sensitivity as film. With three-channel RGB excitation for Cy5/Cy3/Cy2 fluorescent Western blot applications, you can quantify three proteins on a single blot (or two proteins and a loading control) without stripping and re-probing.

The Azure 400 also enables highly sensitive chemiluminescent detection so you can say goodbye to the hassles of film and darkroom chemicals—just place your sample in the system and capture an image with the click of a button.

Key features
  • Three channel RGB excitation for Cy5/Cy3/Cy2 applications-allows quantitation of two proteins (or modifications) and a loading control without stripping and re-probing
  • Fast chemiluminescent detection without the hassles and inconveniences of film
  • Single, multi-functional instrument can image and quantify all of your gels and blots-DNA, protein, colorimetric and photometric
  • Upgradeable to two-channel NIR fluorescent detection to meet future lab needs. The Azure 400 can be field upgraded to the 600 to image samples in the NIR
  • 4.8 OD Dynamic Range
  • Applications include Colour imaging, Blue Light, UV Light, Chemi and RGB.
 

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Azure Biosystems Azure 300

Azure 300


Chemiluminescent Western Blot Imaging System

Get digital chemiluminescent detection at a sensitivity equal to film but without all the hassles. Simply place your Western blot into the system and acquire the image with the click of a button.

Key features
  • Compact design-with a footprint of 38cm W X 55cm H X 36cm D, the small size translates into more room on your bench
  • Fully upgradable-add visible and/or NIR fluorescent detection capabilities as your needs evolve
  • 4.8 OD Dynamic Range
  • Applications Colour Imaging, Blue Light, UV Light and Chemi
 

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Azure Biosystems Azure 200

Azure 200


Gel Imaging Workstation

Easily image DNA and protein gels images without a darkroom with the Azure 200. Use your choice of dyes or stains and the system automatically selects the light source and filters for you—UV for Ethidium bromide-stained DNA gels, blue light for SYBR®Safe or similar dyes, white light for Silver stain or Coumassie Blue.

Key features
  • Compact design-38cm W X 55cm H X 36cm D, keep more space free on your bench
  • Fully upgradable-add fluorescent or chemiluminescent Western detection capabilities as your needs evolve
  • Applications include Colour Imaging, Blue Light and UV Light.
 

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Azure Biosystems AzureSpot Analysis Software

AzureSpot Analysis Software


Advanced Analysis for 1D Gels and Blots

AzureSpot helps you interpret your data easily and accurately. Analyze your gels and blots in either manual or automatic modes. AzureSpot can automatically detect lanes and bands, even on distorted gels, and apply your selected method of background correction. Visualize intensity plots by lane (sample) and channel (probe) to get detailed information about your proteins of interest.

The integrated tutorial helps you get the most information out of your analyses. Generate a PDF report of your experiment when you are done. You can also export your data in many formats to other applications (e.g. Excel) for further analysis.

AzureSpot can
  • Perform 1D densitometry
  • Automatically detect lanes and bands
  • Correct for background
  • Calculate molecular weights
  • Quantify fluorescent signals
  • Annotate your results
  • Visualize multiplex analyses
  • Export reports
Key features
  • Integrated tutorial
  • PDF report generator
  • Lane creation tools
  • Molecular size/pI calibration
  • Quantity calibration toolbox
  • Module for easy shape selection/deselection
  • Wide range of data fields to display in measurements
  • Annotation tools for comments and highlighting of image
Hardware and Software Requirements’

Operating Systems: Windows 10, Windows 8, Windows 7 and Windows Vista Processor 1.4 GHz, Memory Minimum 256MB, recommended 512MB Free Hard Disk Space Recommended 5GB.

 
AzureSpot
 
AzureSpot
 

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Azure Biosystems Azure Cielo - Realtime PCR

Real-time PCR systems: Azure Cielo 3 & Azure Cielo 6


The Azure Biosystems™ Cielo™ 3 and 6 real-time PCR Systems are designed to offer smart protocols, high quality data, robust durability and an intuitive user experience.

Their novel fiber optic detection system allows for enhanced sensitivity and speed. 3 or 6 standard channels let you analyze a variety of commercially available dyes, already in your workflow. Onboard software operated by a 10.2″ touchscreen minimizes footprint and saves you bench space.

Easily connect to your Cielo via Wi-Fi, ethernet, USB or LAN. Get notified after each completed run and even remotely access protocols and files.

Applications
  • Quantitative and qualitative gene expression analysis
  • miRNA analysis
  • Genetic mapping
  • Genetic fingerprinting
  • NGS library quantification
  • 3 or 6 channel multiplex ability
  • Pathogen quantification
Key features
  • Tested durability up to 1000 repeat experiments. 1 year standard warranty
  • Marlow Peltier heating block with 12 gradient temperature zones
  • 6.0 °C/sec ramp rate
  • 96-well format for semi-skirted or un-skirted plates
  • Fiber optic detection for 16-wells simultaneously
  • 3 or 6 optical channels for multiplexing
  • Sensitivity up to 1 copy of amplified DNA, 10 logs of dynamic range
  • Open source chemistry, including FAST. ~20 minute run-time
  • Easily export data via Wi-Fi, ethernet, USB or LAN. Get notified after each completion
Dyes/Probes Azure Cielo 3 Azure Cielo 6
SYBR™ Green, EvaGreen™, FAM™
VIC®, HEX™, JOE™, CAL Fluor® 540, CAL Fluor® Orange 560,
TAMRA™  
ROX™, TEX®615, CAL Fluor® Red 610  
CY®5, Quasar®670, Liz® Mustang Purple®
CY® 5.5  
 

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Photon Etc Photon Etc

Photon etc. is a Canadian designer and manufacturer of highly sensitive infrared cameras, ultrafast hyperspectral imaging (wide-field, microscopy and Raman) ideal for academic research and cutting-edge industrial applications.

Website: http://www.photonetc.com

Photon Etc

IR VIVO™ – NIR-II Fluorescence Imaging

IR VIVO
Opening a new Window into Living Tissues for Preclinical Research

Using near and shortwave infrared, IR VIVO benefits from reduced scattering as well as minimal tissue absorption and auto-fluorescence. This allows deeper, clearer imaging than standard optical imaging. IR VIVO takes advantage of the most recent developments in SWIR imaging. It combines Photon etc.’s ultra-low noise InGaAs camera (Alizé™ 1.7 or ZephIR™ 1.7) with novel illumination and powerful analytical software to provide an unprecedented combination of fast, high resolution and deep imaging.

NIR-II Imaging Properties:
  • High spatial resolution
  • High temporal resolution (real-time dynamics)
  • Non-ionizing & non-invasive
  • Good penetration depth (10x greater than traditional visible optical systems)
.
IR VIVO™
EMISSION SPECTRAL RANGE  

500 – 1620 nm
Extension available in the visible up to 400 nm

FILTER TYPES   Multispectral: Filter wheel
Hyperspectral: HyperCube™
HYPERCUBE SPECTRAL RESOLUTION  < 4 nm
ILLUMINATION SOURCE  

735nm or 808nm laser (1mW/mm²)
Other sources available upon request

ILLUMINATION AREA   15.6 x 12.5 cm field of view for 3 mice model
FIELD OF VIEW   variable from 5 x 4 cm to 15.6 x 12.5 cm
OVERALL INSTRUMENT   DIMENSIONS  

Tabletop
About 75 x 115 x 75 cm

STAGE TEMPERATURE   Up to 40ºC
ANESTHETIC TUBING AND   NOSECONE   3-mice anesthetic gas manifold supplied
COMPUTER   PC with Windows 7-64 bits included
IMAGING SOFTWARE   PHySpec™ software
SPECTRAL DATA FORMAT  HDF5, FITS
SINGLE IMAGE DATA FORMAT  HDF5, CSV, JPG, PNG, TIFF
   
POWER REQUIREMENT   120 VAC / 12 A / 60 Hz
ACQUISITION MODE AVAILABLE   Unfiltered (Broadband)
VIDEO MODE   Megapixel camera for sample visualization

—-  CAMERA  —-

TYPE   InGaAs (Alizé™ 1.7 or ZephIR™ 1.7)
FPA  

640 x 512 pixels

PIXEL SIZE   15 µm
QE   < 75%
DYNAMIC RANGE   13/15 bits

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Applications:

The increased penetration depth and contrast of NIR-II imaging combined with its fast acquisition speed and micron-scale spatial resolution enable to visualize simultaneously microvasculature and blood flow through an intact cranial bone. Other biological applications include pharmacokinetics of chemotherapy drugs and lipid quantification in liver and blood circulation.

Publications:
Real Time In Vivo Imaging IF ICG in the NIR-II with IR VIVO™ Imaging System
Real Time In Vivo Imaging

They sought to develop a near infrared II small animal imaging system which could provide real time images and videos of shortwave IR (SWIR) fluorescent signals in vivo at wavelengths over 1000 nm. It was hypothesized that the SWIR wavelengths would give optimal resolution for in vivo optical imaging due to the low tissue auto fluorescence, scattering and absorption of light at these wavelengths. The desired preclinical imaging system should enable measurement of heart rate, respiratory rate, hepatic function, hepatobiliary and intestinal function, blood flow and angiography in small animals.

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Using Pre-Clinical Imaging to Detect Cancer

In this interview, Émilie Beaulieu Ouellet talks about the recently released and breakthrough imaging system: the IR VIVO™ and its technology. Photon etc.’s IR VIVO™ system is the first and only turnkey hyperspectral preclinical imager optimized for imaging in the second biological window of the near-infrared (NIR-II) / short-wavelength infrared (SWIR) range available on the market to our knowledge. NIR-II imaging will bring an unprecedented combination of fast, high resolution and penetration depth imaging at lower cost and to a broader community than current preclinical imaging techniques. Altogether, it will enable to resolve and track single biomedical targets or processes throughout small animals, thereby opening a new window of possibilities for fundamental and biopharmaceutical research.

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Real-time bio distribution and pharmacokinetics of ICG in a mouse using NIR-II fluorescence