Tomocube – 3D Holographic Microscopy
Tomocube are pioneers of Holographic Microscopy and their ‘HT’ microscopes allow users to quantitatively and noninvasively investigate live biological cells and thin tissues in 3D. This technology works by reconstructing the 3D refractive index (RI) distribution of live cells, and it provides structural and chemical information about the cell, including dry mass, morphology, and dynamics of the cellular membrane. These instruments cater to a number of applications in the fields of Haematology, Cell Biology, Immunology, Microbiology and Nanotechnology.
HT-X1: Multiplying Knowledge from 2D to 4D and Beyond
Observations of cellular morphology and activity are typically based on labeling of target molecules. These methods are invasive, affect the nature of the target molecules and potentially interfere with their biological relevance. In addition, these forms of label are not effective in providing quantitative information. The tools used to visualize labels are generally damaging to cells as often laser-based illumination is used to excite fluorochromes which in turn causes phototoxic damage to the cells.
The "holy grail" of imaging is to visualize cells without labeling to ensure the cells behave and grow normally. Holotomography (HT) does this by capturing the intrinsic light scattering properties of cellular materials using very low levels of light intensity, just enough to allow the light to pass through the cell. In doing so, the refractive index (RI) information of structures within the cell can be collected and selectively pseudo-colored to reveal the cell and its organelles.
In capturing the RI distribution, the Tomocube HT-X1 Holotomography can also provide quantitative data in 3D such as the volume, surface area, and dry mass of cells and their intracellular structures.
- High resolution, high contrast, and high sensitivity
- Large captured fields
- Programmable multi-well plate imaging
- Laser autofocus for well-to-well reproducibility
- Low noise, speckle-free imaging with no calibration step
- Bioimaging made quantitative
- Integrated incubation for long term timelapse
- Integrated 4-channel FL light engine (Blue, Green, Red, Far red)
- Correlative holotomography with 3D fluorescence
- True multi-dimensional imaging across time, space and modality
HT-1: Revolutionary Holotomographic Microscopy
Cellular analysis plays a crucial role in a wide variety of research and diagnostic activities in the life science. However, the information available to researchers and clinicians is limited by current microscopy techniques. An innovative new tool – Holotomographic microscopy – can overcome many of these limitations and open new vistas for researchers and clinicians to understand, diagnose and treat human diseases.
Holotomographic Microscopy – New era of microscopy Tomocube’s holotomography series utilize optical diffraction tomography (ODT), which enables users to quantitatively and noninvasively investigate biological cells and thin tissues. ODT reconstructs the 3D refractive index (RI) distributions of live cells and by doing so, provides structural and chemical information about the cell including dry mass, morphology, and dynamics of the cellular membrane.
- 3-D Refractive Index: new imaging contrast
- High resolution
- No labelling
- Low laser
- Fast in 2D/3D
- High Impact no cost
HT-2: Holotomographic microscopy with 3D fluorescence imaging capability
The HT-2 from Tomocube combines both holotomography and 3D fluorescence imaging into one microscope. The HT-2 facilitates three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination with minimal stress on cells. This technology opens the door to a variety of previously unexplored applications in the fields of bioscience and life science and enables researchers and clinicians to break into new frontiers of understanding, diagnosing, and treating disease.
- A three-channel LED light source (385 nm, 470 nm, 570 nm)
- Z-stack images with a motorized Z-drive (step resolution: 150 nm)
- Correlative analysis in 2D, 3D and 4D with HT and fluorescence images
- Upgradable: The HT-1 can be upgraded easily to the HT-2(fluorescence version) in the field
- Live cell molecular and holographic imaging with minimal stress on cells