Light Sheet Microscopy
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Applied Scientific Instrumentation, Inc. (ASI) were stablished in 1990. Their product line of devices for microscope automation and imaging represents the best technology available. ASI develops Ultra Precise Motion Control Systems, Automation, Modular Microscopes, Complete Light Sheet Systems, and components, Imaging Accessories, Focusing Systems, and High Precision Motorized Stages.
Website: http://www.asiimaging.com/
Dual Inverted Selective Plane Illumination Microscopy (diSPIM)
ASI has developed a new form of light sheet microscopy with our collaborators within the scientific community. The system utilizes two water emersion objectives mounted at 45 degree angles above the sample on an inverted microscope, a third objective also can be used to view the sample from below. The top objectives are mounted on piezo positioning devices so precise image stacks can be obtained. The system utilizes micro-mirrors to generate the light sheet, and the complete system can be mounted on to an existing inverted microscope, or supplied with ASI’s Rapid Automated Modular Microscope system (RAMM). Some of the benefits of the system include:
- Use conventional mounting /glass cover slips
- Generate 3D volumes with isotropic resolution (330 nm in all directions)
- Axial resolution is ~2x better than confocal- or spinning disk systems
- Achieve a ~7-10 fold reduction in photobleaching
- Acquisition rates up to 200 images per second or 2-5 volumes per second (roughly 10x faster than spinning-disk microscopes)
- Compared to Bessel beam plane illumination approaches, diSPIM offers equivalent (or better) axial resolution (~330 nm), 10-100x faster volumetric imaging rates (0.5-1 s instead of 10-100 s), and the ability to image over ~10x more time points (~1000 instead of ~100), due to the significantly lower illumination dose employed in our experiments and the lack of extraneous illumination outside the focal plane
- Microscope has multicolor capability, and has been tested successfully on cells cultured on cover slips, cells embedded in collagen gels, and nematode and zebrafish embryos.
For further information about diSPIM, check out the following link
https://www.ibiology.org/talks/selective-plane-illumination/
iBiology.com Microscopy Course about diSPIM presented by Dr Hari Shroff
Luxendo was founded in 2015 as spin-off from the European Molecular Biology Laboratory (EMBL). They develop and manufacture proprietary light-sheet fluorescence microscopy instruments. With a strong focus on applications, LUXENDO’s solutions are easy to use. The microscopes avoid sample photo toxicity by sequentially illuminating a stack of thin slices of the organism, allowing scientists to observe living organisms for extended periods of time without photo damage.
Website: https://luxendo.eu/
TruLive3D Imager
The Inverted Dual-Sided Illumination Light-Sheet Microscope
The new TruLive3D Imager from Luxendo is optimised for fast 3D multi-sample imaging of delicate live specimens in their native 3D environment. The optical concept, with dual-sided illumination and single-lens detection from below, enables fast acquisition speed, high-resolution imaging, and minimal shadowing effects. The system is especially suited for multi-position imaging of small embryos (e.g. Zebrafish, Drosophila or mouse), 3D spheroids, oocytes and more, enabling time-lapse, high-throughput imaging experiments.
Key Features:
- Close-to-natural environmental conditions for sensitive samples
- The NEW ready-to-use TruLive3D Dishes facilitate sample mounting
- Compact, vibration-free and robust design
- Resolution down to 255 nm in xy
- Browser-based user interface
LCS SPIM
The Light-Sheet Microscope for Large Cleared Samples
The LCS SPIM is one of the latest product in LUXENDO’ s growing family of light-sheet microscopes. The basic configuration of the Luxendo LCS offers a cost-effective solution for cleared sample imaging. Based on a very versatile layout, the highly flexible upgrade options expand the performance of the system to increase speed and optical performance.
Sample Chamber:
An exchangeable quartz crystal cuvette simplifies the whole sample mounting procedure as well as cleaning and maintenance, while ensuring compatibility with a wide range of clearing solutions by covering a refractive index (RI) range from 1.33 to 1.57. The cuvette is available in different lengths to accommodate samples of different sizes: the maximum cuvette dimensions are 40 mm × 20 mm × 20 mm (l × w × h).
Key Features:
- High-performance, cost-effective bench-top device
- Unique cuvette-based sample mounting approach
- Fast 3D imaging of large cleared samples
- Compact, vibration-free and robust design
- Resolution down to 1 µm in 3D
- Browser-based user interface
MuVi-SPIM
The Multiview Light-Sheet Microscope
The MuVi SPIM is a horizontal setup designed to image large volumes very fast. The unique 4-axis concept with its two-sided illumination allows four combinations of illumination and detection. This provides four orthogonal views of the specimen without the need for sample rotation, avoiding shadowing effects and facilitating long-term imaging. Combined with the sample mounting from below, this allows maximized acquisition speed and high precision of data fusion while preserving stability and integrity of the sample.The MuVi SPIM capacities can be further extended to enable imaging of optically cleared samples and performing photomanipulation experiments (e.g. ablation, bleaching). For this purpose, the MuVi SPIM CS (Cleared Sample) and the MuVi SPIM PM (Photomanipulation) have been conceived.
Key features:
- Unique 4-axis concept
- 3D imaging of both large life specimens and cleared tissue
- Compact, vibration-free and robust design
- Resolution down to 280 nm in 3D
- Browser-based user interface
MuVi SPIM LS (Live Sample)
The MuVi SPIM LS is the very first light-sheet microscopy system by LUXENDO.
Featuring a horizontal setup, it is designed to image large volumes of living objects very fast. The unique 4-axis concept with its two-sided illumination facilitates four orthogonal views of the sample without the need for rotation.
MuVi SPIM CS (Cleared Sample)
The LUXENDO MuVi SPIM CS is an extension of the MuVi SPIM capabilities to enable 3D imaging of optically cleared samples.
MuVi SPIM PM (Photomanipulation)
The LUXENDO MuVi SPIM PM is an extension of the MuVi SPIM capabilities to enable photomanipulation of your sample.
InVi-SPIM
The Light-Sheet Microscope Optimized for Long-Term 3D Fluorescence Imaging
Dedicated to live imaging, the Luxendo InVi SPIM is a microscope that has been optimized for long-term 3D fluorescence imaging of living specimens. Easy access to the sample chamber, precisely controlled environmental conditions, maximized photo-efficiency, and short illumination times enable long-term imaging without harming live specimens. The minimization of required specimen medium further leverages its functionality. The optical configuration combined with the fast acquisition speed of the InVi SPIM enables 3D reconstruction, tracking of cellular and subcellular positions, and morphological analysis in real time.
Key features:
- Close-to-natural environmental conditions for sensitive samples
- Compact, vibration-free and robust design
- Resolution down to 255 nm in xy
- Browser-based user interface
InVi SPIM
The InVi SPIM is the first commercially available inverted light-sheet microscope.
It is perfectly suited for a great variety of samples and applications ranging from cell culture to whole embryos (e.g. Drosophila and Zebrafish).
InVi SPIM Lattice Pro (Advanced Illumination Module)
The Advanced Illumination Module (AIM) is a modular extension of the InVi SPIM that provides the highest level of flexibility for illuminating your sample with a light-sheet.
It expands the capabilities of the InVi SPIM: while maintaining the ease-of-use and stability of the system, it adds tailorable, interactive adaptability of the beam shape to suit the highly specific requirements of your sample. You choose what gives you the best results for your 3D high resolution imaging experiment – large field of view, high speed or optimal spatial resolution.
QuVi SPIM: The Upright Light-Sheet Microscope
The QuVi SPIM combines dual views with dual detection channels to enable large-scale 3D imaging of life specimens and cleared samples. The system enables fast and simple transition between from living and cleared tissue imaging. Its novel stage design allows using SBS-format plates and provides an easy sample accessibility by means of its quick load feature. Since the objective lenses are exchangeable, a wide variety of samples of different sizes and preparations can be imaged.
Key Feautures
- Easy sample accessibility through quick load feature
- Compact, vibration-free and robust design
- Resolution down to 340 nm in 3D
- Browser-based user interface