Multi-Photon Imaging System
Scientifica Multiphoton Imaging System
Scientifica’s award-winning two-photon imaging system comes with a galvanometer scan head for the greatest flexibility to direct the laser to any position in the field of view.
‘Galvo’ mirrors offer the benefit of variable scanning speeds, adjustable to suit each individual application. Highly flexible scan patterns such as arbitrary line scans, spiral scans or patterned point scanning (e.g. for uncaging experiments) can easily be achieved.
Variable scanning speeds
Capture a specific region, improve speed and increase the amount of data collected. Rather than relying on averaging to improve signal-to-noise ratios, a reduced scan speed can achieve the same cell image morphology results while decreasing a sample’s exposure to laser light. This reduces bleaching and tissue damage.
Outstanding images of the finest structures deep within your sample. Combine with optical sectioning to produce excellent three-dimensional models.
Region of interest scanning
Increase the yield and veracity of your results when studying small cellular structures, such as a spine or bouton, on a dendrite or axon.
Fast frame rates
The galvanometer scan mirrors facilitate frame rates of up to 15 frames per second for freely rotatable fields of view or 30 frames per second with bi-directional scanning.
Pioneering slim design
A compact footprint and flexible design as the basis for the Multiphoton system allows easy integration with other techniques such as electrophysiology.
Easily convert between in vivo and in vitro
The SliceScope’s unique removable sub-stage, increasing the range of experiments which can be carried out on one rig.
Uniform “spot size”
The relay lens system within the Scientifica scan head achieves highly accurate imaging of small structures and prevents vignetting (or loss of signal) at the edge of the image.
|Scanning mirrors||Scanning mirrors |
3 x 5 mm 8315KL scanning mirrors (MicroMax 671HP Drivers)
|Beam input height||Beam input height |
MP-1000: 198 mm
MP-2000: 280.6 mm
|Maximum beam input diameter||Maximum beam input diameter |
|Relay lens expansion||Relay lens expansion |
|Beam expansion||Beam expansion |
|Beam diameter at back aperture||Beam diameter at back aperture |
MP-1000: <10 mm
MP-2000: <20.1 mm
|Lens coating||Lens coating |
700-1200 nm (Rav <0.5%)
|Maximum scan speed||Maximum scan speed |
2 kHz in bidirectional scan mode (4 fps @512×512 px),
1 kHz in unidirectional scanning mode (2 fps @512×512 px)
|Maximum scan angles (galvo)||Maximum scan angles (galvo) |
MP-2000: +/- 10 optical degrees
|Maximum scan angles (scan head)||Maximum scan angles (scan head) |
+/- 7.6 optical degrees
|Galvo voltage/optical degree||Galvo voltage/optical degree |
|Turning mirror size||Turning mirror size |
MP-1000: 26 x 36 x 3 mm
MP-2000: 45 x 64 x 6 mm
|Turning mirror coating||Turning mirror coating |
Protected silver (98% reflectivity)
|Typical field of view||Typical field of view |
MP-1000: ~800 µm2 (16x)*, ~750 µm2 (20x)*
MP-2000: ~300 µm2 (40x)*
|Scan Control||Scan Control |
SciScan, ScanImage 3.8.1
*May not be achievable if scanning at high speeds for prolonged periods of time