Tomocube HT-2 : Holotomographic microscopy with 3D fluorescence imaging

Mar 6, 2019
Category: General
Tomocube HT-2 : Holotomographic microscopy with 3D fluorescence imaging

The HT-2 from Tomocube combines both holotomography and 3D fluorescence imaging into one microscope. The HT-2 facilitates three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination with minimal stress on cells. This technology opens the door to a variety of previously unexplored applications in the fields of bioscience and life science and enables researchers and clinicians to break into new frontiers of understanding, diagnosing, and treating disease.

The HT-2 is able to achieve label-free imaging of cells and allows users to conduct long-term tracking of specific areas within live cells. It has the capacity to deliver correlative analysis in 2D, 3D and 4D with HT and fluorescence images, and incorporates a customisable three-channel LED light source (385 nm, 470 nm, 570 nm). HT-2 generates Z-stack images with a motorised Z-drive (step resolution: 150nm).


Correlative microscopy in one instrument: - HT-2 provides high-quality 3D images of both holotomography and 3D fluorescence for each sample.

Quantitative data marked with fluorescence: - HT-2 provides morphological (volume, surface area, projection area, sphericity and ellipticity), chemical (dry mass, concentration) and mechanical (cell deformability) properties of cells with 3D refractive index (RI) tomogram. Moreover, fluorescence image provides information about molecular specificity.

Live cell molecular and holographic imaging with minimal stress on cells: - Simultaneous measurement capability of time-lapse 3-D RI tomography and fluorescence image allows long-time tracking of specific targets in live cells. The fluorescence image provides the position of specific target organelles or structures in live cell, and consecutive measurements of time-lapse 3D RI tomography enables the monitoring of cells and their structures with minimal stress.

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