Spectroscopy & Hyperspectral Imaging

Cytoviva – Hyperspectral Imaging System

The CytoViva Hyperspectral Imaging System (HSI) provides spectral analysis of materials and biologicals imaged with the CytoViva Nano-scale Optical Microscope. Operating in the visible near infrared spectral range (VNIR), CytoViva HSI supports research in areas such as nano-drug delivery and nano-toxicology. This spectral analysis method supports both non-fluorescent and fluorescently labeled components in live cells and nano-materials.

In each application, the CytoViva Nano-scale Microscope and Hyperspectral Imaging Systems work together as a seamless solution to provide analytical confirmation of nano-scale materials and their interactions with biologicals or other composite materials.

CytoViva HSI combines the following primary components:

  • A concentric imaging spectrophotometer containing original, aberration-corrected convex holographic diffraction gratings. Operating in the 400-1,000nm spectral range, this patented spectrophotometer technology provides superior signal-to-noise ratio insuring that only spectra from the high contrast CytoViva microscope image is captured for analysis.
  • Customized hyperspectral image analysis software specifically designed to support microscopy applications. This proprietary software is a modified version of the ITT Visual Information Solutions ENVI hyperspectral image analysis system. As a data collection and reporting capability, this software is easy to use and highly intuitive. Using this system, spectral analysis can be performed within a user defined region of interest down to the pixel level. This image analysis software enables the researcher to easily locate regions within the sample that match qualified spectra that has been preloaded in a library. It also provides the ability to un-mix spectra to isolate specific sample components or sample anomalies for further evaluation.
  • A motorized stage, integrated onto the CytoViva microscope system, acts as a “push broom”, moving the sample precisely over the hyperspectral image detector field of view. Operation of this push broom capability is integrated into the image analysis software system.

While this system was specifically designed to support nanoscale studies, it also supports a wide range of bio-medical research initiatives in areas such as cell biology and infectious disease.

Cytoviva Download datasheet

 For more information visit:  https://cytoviva.com/products/hyperspectral-imaging-2/hyperspectral-imaging/

CytoViva Hyperspectral Microscope Introductory Video
Optical observation, spectral characterization and mapping of nano-materials in live cells, tissue and other composite materials. No fluorescent markers are required. Advance your work in nano-materials development, nano-medicine and nano-toxicology.

Nano-Scale Hyperspectral Imaging

The images below are representative of a typical application for the CytoViva VNIR Hyperspectral Imaging System in the area of nano-bio research. This application illustrates the ability of the CytoViva system to quantify the presence of 100nm gold nano-particles incubated with a live epithelial cell.

This data was captured through the integrated use of the high contrast CytoViva Microscope, which creates the image; The high signal-to-noise CytoViva Hyperspectral Imager, which captures the spectral image and the CytoViva Hyperspectral Image Analysis Reporting Software.


Optical Image

Live epithelial cell incubated with gold nano-particles; Imaged with CytoViva Microscope System, 40x objective and Dage MTI Excel16 color digital camera. Note the visible gold nano-particles.

Hyperspectral Image Scan

Live epithelial cell incubated and gold nano-particles: Image scanned with CytoViva microscope system, 40x objective and CytoViva Hyperspectral Imaging System.

Red colored area in the sample represents where gold nano-particles are visible.

Yellow colored area in the sample represents an area in the cell where gold particles are not visible.

Spectral Plot

Spectral plot of light scattering from the red colored area of the sample where gold nano-particles are visible. This spectral signature is consistent with previously measured spectra of these nano-particles.

Spectral plot of light scattering from the yellow colored area of the cell sample, where gold nano-particles are not visible.